本研究从5种培养基中筛选出最适于雨生红球藻HP1藻株绿色细胞生长阶段细胞增殖的培养基，并针对筛选出的培养基分别进行有机碳源和无机碳源及其浓度的优化。在此基础上，以光照强度、初始pH值和诱导时间为实验因素，采用单因素试验、中心点复合设计（CCD）响应面法对雨生红球藻虾青素积累阶段的胁迫条件进行优化。结果表明：HP1藻株在配方A及OHM培养基中均能达到较高生物量，有机碳源和无机碳源的最优浓度均为0.5 g/L。HP1虾青素积累的最优胁迫条件为：光照145 μmol/m2·s，初始pH 7.0，诱导时间9 d。在上述条件下，以有机碳源（NaAC）和无机碳源（NaHCO3）为碳源的培养体系中，虾青素产量最大分别为5.74 mg/L、4.25 mg/L。研究结果将为进一步提高雨生红球藻虾青素产量提供科学依据。

In this study, an optimal medium for cell proliferation of Haematococcus pluvialis HP1 during the green cell growth stage was selected from five media. Furthermore, the organic and inorganic carbon and their concentrations in the selected medium were also optimized respectively. Based on this, the single factor experiment and central composite design (CCD) response surface analysis were utilized to optimize the stress condition of astaxanthin accumulation with light intensity, initial pH value and inducement time as experimental factors. The results indicated that the biomass of HP1 could reach a higher level in medium A and OHM, and the optimal concentration of organic carbon source and inorganic carbon source was 0.5 g/L. The optimal stress conditions for HP1 astaxanthin accumulation were: light 145 μmol/m2·s, initial pH 7.0, and induction time 9 d. Under the conditions described above, the maximum yield of astaxanthin reached 5.74 mg/L in NaAC culture system and 4.25 mg/L in NaHCO3 culture system, respectively. This study can provide a scientific basis for further increasing the astaxanthin yield of Haematococcus pluvialis.

四川省科技厅重点研发项目(2020YFN0127)