探讨赶黄草总黄酮对四氯化碳所致小鼠急性化学性肝损伤的保护作用。选取50只SPF级昆明小鼠分为正常组、模型组、联苯双酯滴丸组（150 mg/kg）、赶黄草总黄酮高剂量组和低剂量组（800，400 mg/kg）5个组。各组均灌胃给药，1次/d，连续给药21 d。末次给药1 h后，除正常组外，其余各组均腹腔注射1% CCl4橄榄油溶液（10 mL/kg），16 h后处死小鼠，全自动生化仪检测小鼠血清ALT、AST、ALP和LDH水平，ELISA法检测肝组织TNF-α、IL-6、SOD和MDA水平，HE染色观察肝脏病理组织改变。与模型组比较，赶黄草总黄酮高剂量组血清ALT、AST、ALP和LDH活性分别降低了86.01%、91.03%、25.50%和95.54%，赶黄草总黄酮低剂量组血清ALT、AST和LDH活性分别降低了76.06%、84.91%和93.87%；赶黄草总黄酮高剂量组肝组织TNF-α、IL-6和MDA含量分别降低了24.89%、20.91%和39.45%，而SOD活性升高了48.22%；赶黄草总黄酮低剂量组肝组织IL-6和MDA含量分别降低了18.60%和25.62%，而SOD活性升高了42.23%；赶黄草总黄酮高、低剂量组肝组织病理状态明显改善。赶黄草总黄酮对四氯化碳所致小鼠急性化学性肝损伤具有较好的保护作用，作用机制可能与其抑制氧化应激反应及炎症反应有关。

To study the protective effect of total flavonoids of Penthorum chinense Pursh on acute chemical liver injury induced by carbon tetrachloride in mice, in this study, 50 SPF Kunming mice were divided into five groups including normal group, model group, bifendate dropping pills group (150 mg/kg), total flavonoids of Penthorum chinense Pursh in high and low dose groups (800, 400 mg/kg). The mice were intragastrically administered with drugs or distilled water according to the body weight once a day for 21 days. One hour after the last administration, the other groups except the normal group were treated with the intraperitoneal injection of 1% CCl4 olive oil solution (10 mL/kg). Then all mice were killed after 16h, and the serum was collected for the determination of ALT, AST, ALP and LDH levels by automatic biochemical analyzer. Liver was triturated for detecting TNF-α, IL-6, SOD and MDA by ELISA method. The pathological changes of liver tissue were observed by HE staining. Compared with the model group, the activities of serum ALT, AST, ALP and LDH in high dose group were respectively reduced by 86.01%, 91.03%, 25.50% and 95.54%, while in low dose group were respectively decreased by 76.06%, 84.91% and 93.87%; in high dose group, the contents of TNF-α, IL-6 and MDA in liver tissue were respectively decreased by 24.89%, 20.91% and 39.45%, while SOD activity increased by 48.22%; in low dose group, the contents of IL-6 and MDA in liver tissue were respectively decreased by 18.60% and 25.62%, while SOD activity increased by 42.23%; and the pathological conditions of liver were significantly improved in high and low dose groups. The results suggest that total flavonoids of Penthorum chinense Pursh exhibits the protective effect on acute chemical liver injury induced by CCl4 in mice, and the mechanism of action may be associated with its inhibition of the oxidative stress reaction and the inflammatory reaction.

泸州市人民政府-西南医科大学科技战略合作项目（2019LZXNYDZ14）；四川省中医药管理局中医药科研专项课题（2020CP0029）