比较茯苓不同溶剂提取物改善小鼠的睡眠作用。通过直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验研究茯苓不同溶剂提取物改善小鼠的睡眠作用，通过测定小鼠脑组织中5-HT、GABA和DA含量变化探讨茯苓不同溶剂提取物改善小鼠睡眠作用机制。结果显示，与空白对照组相比，水提物高剂量组（112.7 mg/kg）效果最佳（p<0.05），其后是醇提物高剂量组（100.8 mg/kg）、多糖高剂量组（15.6 mg/kg）、水提物低剂量组（56.4 mg/kg）、醇提物低剂量组（50.4 mg/kg），而多糖低剂量组（7.8 mg/kg）效果不明显（p>0.05），其中，水提物高剂量组小鼠睡眠时间由1829.67 s显著延长至2979.83 s，睡眠发生率由10%显著提高至60%，睡眠潜伏期由2033.50 s显著缩短至1486.25 s；且茯苓不同溶剂提取物改善小鼠睡眠的效果与其调节小鼠脑组织中GABA和5-HT含量升高及DA含量下降的趋势相一致，其中，水提物高剂量组5-HT含量升至26.96 pg/mg，GABA含量升至817.40 pg/mg，DA含量降至3.41 pg/mg。本研究结果显示水提物高剂量组改善睡眠效果最佳，其作用机制与调节小鼠脑中GABA、5-HT和DA含量有关。

The extent of the improvements in sleep habits of mice treated with Wolfiporia extensa extracts obtained using different solvents were compared using sleep tests, prolonged pentobarbital-induced sleeping time measurements, below-threshold dose pentobarbital-induced hypnosis tests, and pentobarbital-induced sleep latency tests. Changes in the 5-HT, GABA, and DA content in mouse brain tissues were monitored to explore the mechanism by which Wolfiporia extensa extracts obtained using different solvents improve sleep in mice. The results reveal that, the group treated with high-dose aqueous extracts (112.7 mg/kg) show the greatest improvement in their sleep (p<0.05) compared to the blank control. This group is followed by the groups treated with high-dose ethanol extracts (100.8 mg/kg), high-dose polysaccharides (15.6 mg/kg), low-dose aqueous extracts (56.4 mg/kg), and low-dose ethanol extracts (50.4 mg/kg). Analysis also revealed that low-dose polysaccharides (7.8 mg/kg) (p>0.05) treatment induced very little change in the sleep habits of these animals. More specifically, the sleeping time of the mice treated with high-dose aqueous extracts increased remarkably from 1829.67 s to 2979.83 s, and the incidence rate rises from 10% to 60%. In addition, sleep latency was markedly reduced, from 2033.50 s to 1486.25 s, and the sleep improvements associated with these Wolfiporia extensa extracts were shown to coincide with increases in the GABA and 5-HT content and reductions in the DA content in the brain tissues of treated animals. The 5-HT and GABA content reached up to 26.96 pg/mg and 817.40 pg/mg, respectively, in the animals treated with high-dose aqueous extracts while the DA content dropped to 3.41 pg/mg. This data reveals that high-dose aqueous Wolfiporia extensa extracts are the best option for improving sleep in mice, and that this improvement is closely associated with the regulation of GABA, 5-HT, and DA in the brain.

安徽省科技重大专项（18030801112）