探究沉香提取物对PCPA（P-chlorophenylalanine，PCPA）致失眠大鼠的镇静催眠作用。选取SD大鼠90只，10只作为空白组，余80只建立失眠大鼠模型，分为模型组、沉香挥发油高剂量组、沉香挥发油低剂量组、沉香醇提物高剂量组、沉香醇提物低剂量组、沉香水提物高剂量组、沉香水提物低剂量组、地西泮组。观察各组下丘脑5羟色胺（5-HT）、多巴胺（DA）、去甲肾上腺素（NE）、γ-氨基丁酸（GABA）、谷氨酸（Glu）含量及GABAARα1、GABAARγ2、5-HT1A受体mRNA相对表达量。给药6 d后，挥发油高剂量组、挥发油低剂量组、醇提物高剂量组、醇提物低剂量组下丘脑5-HT含量分别为4.85 ng/mL、4.84 ng/mL、4.81 ng/mL、4.49 ng/mL，GABA含量分别为1.46 ng/mL，1.44 ng/mL，1.41 ng/mL，1.39 ng/mL，高于模型组（p<0.05）；挥发油高剂量组、挥发油低剂量组、醇提物高剂量组5-HT1A受体mRNA相对表达量为0.81、0.79、0.74，高于模型组（p<0.05）；GABAARα1 mRNA相对表达量分别为0.94、0.84、0.85，GABAARγ2 mRNA相对表达量分别为0.74、0.70、0.73，高于模型组（p<0.05）。沉香提取物对PCPA致失眠大鼠具有一定镇静催眠作用。

To explore the sedative and hypnotic effects of agarwood extract on PCPA (P-chlorophenylalanine, PCPA) - induced insomnia rats. Ninety SD rats were selected, 10 were used as the blank group, and the remaining 80 rats were used to establish insomnia rat models. They were divided into model group, agarwood volatile oil high-dose group, agarwood volatile oil low-dose group, agarwood alcohol extract high-dose group, and agarwood alcohol the low-dose extract group, the high-dose group of water extract of agarwood group, the low-dose group of water extract of agarwood group, and the diazepam group. Hypothalamic serotonin (5-HT), dopamine (DA), norepinephrine (NE), γ-aminobutyric acid (GABA), glutamic acid (Glu) content and GABAARα1, GABAARγ2, 5-HT1A relative expression of body mRNA were observed. After 6 days of administration, the 5-HT contents of hypothalamus in the high-dose volatile oil group, low-dose volatile oil group, high-dose alcohol extract group, and low-dose alcohol extract group were 4.85 ng/mL, 4.84 ng/mL, 4.81 ng/mL, 4.49 ng/mL, respectively. GABA contents were 1.46 ng/mL, 1.44 ng/mL, 1.41 ng/mL, 1.39 ng/mL, respectively, which were higher than the model group (p<0.05). The relative expression levels of GABAARα1 mRNA in the high-dose volatile oil group, low-dose volatile oil group, and high-dose alcohol extract group were 0.94, 0.84, 0.85, respectively, all of them were higher than the model group (p<0.05). The relative expression of GABAARγ2 mRNA were 0.74, 0.70, 0.73, respectively, and the relative expression of 5-HT1A receptor mRNA were 0.81, 0.79, 0.74, respectively, all of them were higher than the model group (p<0.05). Agarwood extract has a certain sedative and hypnotic effect on PCPA-induced insomnia rats.

中国高等教育学会专项课题重点项目（2020JXD08）