本文研究了微生物数码显微培养计数系统在微生物培养过程中的菌落判定和计数能力。以大肠杆菌、金黄色葡萄球菌、白假丝酵母菌和巴西曲霉四种微生物为测试菌株探讨了传统目测计数方法和仪器计数方法的一致性，并分析了当样品有杂质时对仪器读数的影响。结果显示仪器读数和目视读数具有显著的正相关性（R2=0.85~0.99，p<0.05），该仪器在微生物生长过程中能够实现实时监测，当菌落总数超标时能及时起到预警作用，且仪器能显示出培养皿上各点的三维图像，仪器读数结合人工判断，能较好地区分菌落和杂质，接种回收率在105%~155%之间。在实际固体粉末测试样品中应用，结果显示仪器读数和目视读数接近，一致性比例为94%，且仪器读数更为敏感。因此，微生物数码显微培养计数系统可以很好的应用于食品、药品和化妆品中菌落总数的快速准确定量检测。

The capabilities of the microbial colony counting system (MCCS) including colony determination and counting abilities were examined in this study. Four different microorganisms including Escherichia coli, Staphylococcus aureus, Canidia albicans and Aspergillus brasiliensis were used as testing strains to examine the consistency of the traditional visual counting method and the instrumental counting method, and the influence of impurities in a sample on instrumental reading. The results showed that there was a significant (R2=0.85~0.99, p<0.05) positive correlation between the instrument readings and visual counting results. The instrument can realize real-time monitoring during the growth of microorganisms, and when the total number of colonies exceeds the limit, provides early warning displays 3D images of each point on the petri dish. The instrumental reading combined with manual judgment can distinguish better colonies and impurities and allow an inoculation recovery rate of 105%~155%. When the MCCS system was applied to actual powdered test samples, the result showed that the instrumental readings were close to the visual readings with the consistency rate of 94%, and the instrument was even more sensitive. Therefore, the MCCS can be well applied to the rapid and accurate quantitative detection of the total number of colonies in food, pharmaceutical and cosmetic areas.

国家自然科学基金资助项目（31972062）；广东省科技计划项目（2018A050506008；2016A010105003）；广州市科技计划项目（201903010056）