本文以油塔子为研究对象，通过不同防腐剂的添加量、紫外杀菌时间和紫外杀菌距离考察各因素对菌落总数、霉菌/酵母菌、大肠杆菌数量、色差和感官评分的影响。利用正交试验确定最佳杀菌工艺为：0.012%的Nisin添加量、紫外杀菌45 min，距离为10 cm。此工艺条件处理后，结果表明：冷藏条件下，实验组油塔子在保藏19 d时，霉菌/酵母菌数量为2.85 log（cfu/g），超过国家标准限值2.18 log（cfu/g），而对照组油塔子在保藏9 d时，霉菌/酵母菌数量达到了2.28 log（cfu/g），为腐败油塔子；常温保藏条件下，实验组在第5 d时霉菌/酵母菌超标，而对照组第3 d时就测得其霉菌/酵母菌超过国家标准数量，为2.43 log（cfu/g）。本试验的复合杀菌处理将油塔子的货架期在常温条件下延长到4 d，在冷藏条件下延长到17 d，为油塔子工业化提供了理论依据。

In order to explore the effects of different treatments (different antiseptics, UV sterilization time, UV sterilization distance) on the quality of Youtazi (aerobic bacteria, moulds/yeasts, Escherichia coli count, color difference and sensory evaluation), single factor and orthogonal experiments were employed to determine the optimal parameters. The results showed that the optimum sterilization conditions were Nisin addition 0.012%, sterilization time 45 min and sterilization distance 10 cm. At -4 ℃, the amount of mold/yeast bacteria reached 2.85 log (cfu/g) after 19 days of storage in the experimental groups, which exceeded the national standard limit of 2.18 log (cfu/g), while the amount of mold/yeast bacteria reached 2.28 log (cfu/g) only after 9 days in the control group; in ambient temperature, the amount of mold/yeast bacteria exceeded the standard limit on the 5th days in the experimental groups, while in the control group the amount of mold/yeast bacteria was 2.43 log (cfu/g) on the 3rd day, which exceeded the national standard already. The storage of Youtazi in ambient temperature and -4 ℃ were prolonged to 4 days and 17 days, respectively. This research could provide theoretical basis for the industrialization of Youtazi.

国家重点研发计划重点专项项目（2018YFD0400103-1）