本文研究了人参多糖对冠心病大鼠线粒体的保护作用。通过建立冠心病大鼠模型，采用人参多糖进行干预，检测线粒体钙（Ca2+）、琥珀酸脱氢酶（SDH）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）的含量水平，以及可溶性Fas（FAS）、Fas配体（FASL）、B淋巴细胞瘤-2（Bcl-2）及B淋巴细胞瘤-2基因相关X蛋白（Bax）的表达。结果表明，随着人参多糖作用浓度的增加，Ca2+浓度和SDH活性均有显著性变化（p<0.05），其中，高剂量组Ca2+浓度、SDH活性分别为43.41 μmol/g和7.21 U/mg。人参多糖作用后，高剂量组MDA含量、GSH-Px酶活和SOD酶活分别为4.19 nmol/mL、132.42 U/mg和90.18 U/mL，与模型组均有显著性差异（p<0.05）。同时，与模型组相比，人参多糖能显著降低大鼠的FASL、FAS、Bcl-2及Bax表达水平（p<0.05）。其中高剂量组的FASL、FAS、Bcl-2及Bax的表达量分别为1.21、1.09、1.08和1.02。结果说明，人参多糖可改善冠心病模型大鼠血脂异常，具有抗氧化、抗肿瘤的作用，对线粒体的保护作用较强。

The protective effect of ginseng polysaccharide on mitochondria in rats with coronary heart disease was studied. The levels of mitochondrial calcium (Ca2+), succinate dehydrogenase (SDH), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), soluble Fas (FAS), Fas ligand (FASL), b-lymphoma-2 (Bcl-2) and b-lymphoma-2 genes related X protein (Bax) were detected by the intervention of ginseng polysaccharide. The results showed that the concentration of Ca2+ and SDH activity were significantly changed with the increase of the action concentration of ginseng polysaccharide (p<0.05), among which, the Ca2+ concentration and SDH activity in the high dose group were 43.41 μmol/g and 7.21 U/mg respectively. After the action of ginseng polysaccharide, MDA content, GSH PX enzyme activity and SOD activity in high dose group were 4.19 nmol/mL, 132.42 U/mg and 90.18 U/mL respectively, which were significantly different from those in the model group (p<0.05). At the same time, compared with the model group, ginseng polysaccharide significantly reduced the expression levels of FASL, Fas, Bcl-2 and Bax in rats (p<0.05). The expression of FASL, Fas, Bcl-2 and Bax in high dose group was 1.21, 1.09, 1.08 and 1.02, respectively. The results showed that ginseng polysaccharide could improve the blood lipid abnormality in rats with coronary heart disease, and had the function of anti-oxidation and anti-tumor, and had strong protective effect on mitochondria.

浙江省自然科学基金项目（LQ20H270017）