[关键词]
[摘要]
为研究牛蒡提取物对兔皮明胶膜的改性效果,制备了牛蒡提取物体积浓度为0、2%、4%、6%、8%的牛蒡提取物/兔皮明胶复合膜,测定了各组复合膜的物理性质、抗氧化性、抑菌性,并作结构表征。结果显示:随着牛蒡提取物浓度增加,复合膜膜水蒸气透过滤、水溶性逐渐降低,浓度为8%时最低,为2.22×10-8 g/(m·s·Pa)、19.22%,比对照组下降14.94%、27.56%,抗拉强度、厚度增加,最大为67.11 MPa、74.16 μm,比对照组增加21.66%、12.14%,断裂伸长率先增加后降低,浓度为4%最大为4.86%,比对照组高63.63%;复合膜的透光度明显降低,对紫外线的透光度显著降低,在波长400 nm处吸光最强,透光率为0.003%,雾度显著提升,最大为37.59%;复合膜羟基自由基清除率明显提升,最高为63.53%;复合膜抑菌性逐渐增强,对不同菌体抑菌能力为枯草芽孢杆菌>金黄色葡萄球菌>大肠杆菌>黑曲霉菌;红外光谱和扫描电镜分析发现复合膜形成氢键,结构完整,牛蒡提取物促进了明胶蛋白肽链的展开和伸展。综上可知,牛蒡提取物可以有效提升兔皮明胶膜的性能。
[Key word]
[Abstract]
In order to study the modification effect of Arctium lappa extract on rabbit skin gelatin membrane, Arctium lappa extract / rabbit skin gelatin composite membrane with the volume concentration of 0, 2%, 4%, 6%, 8% was prepared. The physical properties, antioxidative and bacteriostatic properties of each composite membrane were determined and its structure was characterized. The results showed that with the increase of burdock extract concentration, the water vapor filtration and water solubility of the composite membrane decreased gradually. When the concentration was 8%, the lowest values were 2.22×10-8 g/(m·s·Pa) and 19.22%, which were 14.94% and 27.56% lower than those of the control group, respectively. The tensile strength and thickness increased, and the maximum values were 67.11 MPa and 74.16 μ m, increased by 21.66% and 12.14% respectively, compared with the control group. The elongation at break first increased and then decreased, the concentration was 4% and the maximum was 4.86%, which was 63.63% higher than the control group. The light transmittance of the composite membrane was significantly reduced, and the light transmittance of the ultraviolet was significantly reduced, at the wavelength of 400 nm, the light absorption was the strongest, the transmittance was 0.003%, and the haze was significantly increased, the maximum was 37.59%. The clearance rate of hydroxyl radical of composite membrane was significantly increased, the maximum was 63.53%. The antibacterial ability of composite membrane was gradually enhanced, and the antibacterial abilities of composite membrane to different bacteria were ordered as: Bacillus subtilis > Staphylococcus aureus > Enterobacteriaceae > Aspergillus niger. The inhibition ability of Aspergillus niger increased gradually with the increase of the concentration of Arctium lappa extract, and the hydrogen bond formation and crystal formation of the composite membrane were found by infrared spectrum and scanning electron microscope analysis. Burdock extract promoted the unfolding and stretching of gelatin peptide chains. In summary, burdock extract can effectively improve the performance of rabbit skin gelatin film
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[基金项目]
国家自然科学基金项目(31300474)