以侗族酸肉、苗族酸肉两种酸肉为原料，采用木瓜、碱性、风味及中性蛋白酶四种蛋白酶进行酶解，测定酶解液短肽得率、羟自由基及DPPH自由基清除率，结果表明碱性蛋白酶酶解液的短肽得率（侗族86.01%，苗族82.52%）、羟自由基（侗族87.48%，苗族79.88%）及DPPH自由基（侗族74.63%，苗族87.87%）清除率最高。通过比较分析苗族酸肉较侗族酸肉短肽得率更高，因此选择以蛋白酶种类、加酶量、酶解时间及料液比为自变量的单因素试验基础上，以苗族酸肉短肽得率及DPPH自由基清除率为评价指标，采用响应面优化最佳酶解条件。结果表明，酸肉抗氧化肽最佳酶解工艺为:碱性蛋白酶添加量12600 U/g、酶解时间1 h、料液比1:1.09（m:V）。在此最优条件下酶解获得的抗氧化肽得率为83.35%，是预测值的98.99%，DPPH自由基清除率力为84.01%，是预测值的97.33%，与预测值基本一致，表明以碱性蛋白酶酶解的酸肉肽具有较高的抗氧化活性及营养价值，同时为酸肉抗氧化肽的开发及利用提供理论依据。

Dong sour meat and Miao sour meat are used as raw materials, papaya, alkaline, flavor and neutral protease were used for enzymolysis, and the yield of short peptide, hydroxyl radical and DPPH radical scavenging rate of enzymolysis liquid were determined. The results showed that the short peptide yields from alkaline protease enzymolysis were 86.01% (Dong) and 82.52% (Miao), hydroxyl free radical clearance rates were 87.48% (Dong) and 79.88% (Miao), and DPPH free radicals clearance rates were 74.63% (Dong) and 87.87% (Miao). Through comparative analysis, the yield of short peptides from Miao sour meat was higher than that from Dong sour meat. Therefore, protease species, enzyme dosage, enzymatic hydrolysis time and solid-liquid ratio were selected as independent variables, taking the yield of Miao sour meat short peptide and DPPH radical scavenging rate as evaluation index, the optimal enzymatic hydrolysis conditions were determined using response surface. The results showed that the optimal enzymatic hydrolysis process of the antioxidant peptide of sour meat was as follows: the addition of basic protease is 12600 U/g, the enzymatic hydrolysis time is 1 h, and the ratio of feed to liquid is 1:1.09 (m:V). The yield of antioxidant peptide under the optimal conditions was 83.35%, which was the predictive value of 98.99%. DPPH free radical clearance rate was 84.01%, which was the predictive value of 97.33%. This suggests that the peptides from alkaline protease enzymolysis of sour meat have higher antioxidant activity and nutritional value, which provide theoretical basis for further development and utilization.

“十三五”国家重点研发计划重点专项（2018YFD0401200）；河南省高校创新人才计划项目（18HASTIT036）；河南省科技攻关项目（192102110216）；国家现代农业产业技术体系建设专项（CARS-37）