[关键词]
[摘要]
开发一款以茯砖茶为主要原料,以山楂、洋甘菊、陈皮等药食同源的植物材料为辅料的复合茯砖袋泡茶,通过正交试验和感官评价得到最佳配方。以小鼠急性毒性试验为基础,采用Ames试验、哺乳动物红细胞微核试验和体外哺乳动物类细胞染色体畸变试验的遗传毒性试验对其进行安全性评价。结果表明,通过正交试验优化得到的最佳配方为山楂13.16%、蒲公英根13.16%、茯砖茶26.32%、薄荷叶15.79%、小茴香7.89%、陈皮13.16%、洋甘菊10.53%,且内含成分黄酮类4.83%、多酚11.59%、氨基酸4.12%、水浸出物37.56%,稍高于正交试验中得分前5的组合。复合茯砖袋泡茶对小鼠的最大耐受剂量大于21.285 g/(kg·bw),相当于人体推荐剂量的507倍,属于无毒级。此外,在加与不加S9混合液的条件下,Ames试验和体外哺乳类动物染色体畸变试验的结果均是阴性(p>0.05),复合茯砖茶提取物对小鼠骨髓嗜多染红细胞也无致微核作用。说明本复合茶配方无遗传毒性,可放心饮用。
[Key word]
[Abstract]
A compound fuzhuan tea bag was developed by using fuzhuan tea as the main raw material, with other medicinal and edible homologous plant materials such as hawthorn, chamomile and dried tangerine peel as the excipients. The optimal formula was obtained through orthogonal tests and sensory evaluation. The safety of the compound tea was assessed on the basis of acute toxicity tests, and through genetic toxicity tests including the Ames test, mammalian erythrocyte micronucleus test and in vitro mammalian cell chromosome aberration test. The results showed that the optimum formula was obtained through orthogonal experiments: 13.16% hawthorn, 13.16% dandelion, 26.32% fuzhuan tea, 15.79% mint leaves, 7.89% fennel, 13.16% tangerine peel and 10.53% chamomile. The optimal formula contained flavonoids 4.83%, polyphenols 11.59%, amino acids 4.12%, water extracts 37.56%, which were slightly higher than those of the combinations with the top 5 scores in the orthogonal test. The maximum tolerated dose of the compound fuzhuan tea bag in mice was higher than 21.285 g/ (kg·bw), which was 507 times as much as the recommended dose for humans, thus indicating non-toxicity. In addition, the results of the Ames test and in vitro mammalian chromosome aberration tests were negative (p>0.05) in the absence and presence of S9 mixture. The extract of the compound fuzhuan tea had no micronucleus-inducing effect on polychromatic erythrocytes of bone marrow in mice. These results showed that the compound fuzhuan tea had no genotoxicity and is safe for intake.
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[基金项目]
国家重点研发计划项目(2018YFC1604403);湖南主要茶类优质化加工技术研究与新产品开发(2018NK2035);长沙市科技局国际合作与交流项目(kq1706109);都匀毛尖茶叶无公害生产技术创新团队(黔教合人才团队字[2014]45)