[关键词]
[摘要]
龙葵果被广泛用于解热和利尿。本文研究了黑果龙葵(SNL)、黄果龙葵(SDL)和红果龙葵(SAM)三种龙葵果的石油醚(PE)、乙酸乙酯(EtoAc)、正丁醇(n-BuOH)萃取物体外抗氧化和抗炎能力。分别以DPPH·清除试验和二价铁离子还原能力(FRAP)探讨了体外抗氧化能力,以抗坏血酸(Vc)作为阳性对照;并以透明质酸酶和脂氧合酶抑制能力评价其抗炎效果,分别以双氯芬酸钠和去甲二氢愈创木酚作为阳性对照。三种龙葵果不同溶剂萃取物中,黑果龙葵乙酸乙酯萃取物抗氧化能力最强,DPPH·清除试验IC50为119.43 μg/mL,FRAP为2.674 mM FeSO4/L;红果石油醚萃取物抗炎效果最好,透明质酸酶和脂氧合酶抑制IC50分别为810.67 μg/mL和781.28 μg/mL。黑果龙葵中总酚含量最高为27.16 mg/g。红果龙葵中黄酮含量最高为18.59 mg/g。这一研究为天然抗氧化剂和抗炎剂的开发提供了理论依据。
[Key word]
[Abstract]
Solanum nigrum L. berries have been widely used for anti-pyretic and diuretic in China. Antioxidant and anti-inflammation activities in vitro of extracts of Solanum nigrum Linnaeus (SNL), Solanum diphyllum Linnaeus (SDL) and Solanum alatum Moench (SAM) berry which were prepared by petroleum ether (PE), ethyl acetate (EtoAc) and n-butanol (n-BuOH). The antioxidant activites were evaluated by FRAP and DPPH· scavenging assays. Vitamin C was used as positive control. The results showed that DPPH· scavenging IC50 and FRAP values of SNL-EtoAc extract were 119.43±1.19 μg/mL and 2.674±0.071 mM FeSO4/L, respectively, indicating that it had the highest antioxidant activity. The anti-inflammation effect of different extracts was determined by hyaluronidase and lipoxygenase inhibition. SAM-PE extract had the highest anti-inflammation activity, which IC50 were 810.67±79.02 and 781.28±28.01 μg/ml, respectively. Diclofenac sodium and 4,4'-(2,3-Dimethyltetramethylene) dipyrocatechol were used as anti-inflammatory positive control. SNL had the highest content of total phenols (27.16±0.14 mg/g). SAM had the highest content of total flavonoids (18.59±0.14 mg/g). The results provided a theoretical supporting for developing natural antioxidant and anti-inflammatory resources.
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[基金项目]
中央高校基本科研业务费专项项目(2572018BA07)