[关键词]
[摘要]
本文研究了益生菌干预对高脂血症大鼠粪便短链脂肪酸含量的作用规律。复制高脂血症大鼠模型,利用实验室保藏的具有较好降胆固醇效应的Lactobacillus plantarum 67、Lactobacillus fermentum 83,以Lactobacillus rhamnosus LGG为阳性对照,结合“5:2轻断食法”,对模型大鼠进行一周五次的益生菌干预,干预6周后测定粪便中六种短链脂肪酸的含量。与模型组相比,Lactobacillus plantarum 67干预后,丁酸和戊酸含量分别增加了37.97%和41.22%(p<0.05),丙酸含量增加了1.25%(p>0.05),乙酸含量降低了3.19%(p<0.05),异丁酸和异戊酸的含量分别降低了2.11%和14.16%(p>0.05);混合菌株干预后,丙酸、丁酸、戊酸和异丁酸含量分别增加了31.25%、70.92%、103.98%和24.48%(p<0.05),乙酸含量降低了23.38%(p<0.05),异戊酸含量降低7.38%(p>0.05);与阳性对照组相比,Lactobacillus plantarum 67能显著提高乙酸、异丁酸和异戊酸含量,分别提高了31.69%、40.33%和82.83%(p<0.05),戊酸含量提高12.74%(p>0.05),丙酸含量降低了23.58%(p<0.05),丁酸含量降低2.38%(p>0.05);混合菌株能显著增加乙酸、丁酸、异丁酸、戊酸和异戊酸含量,分别增加4.23%、20.37%、78.44%、62.84%和109.48%(p<0.05),丙酸含量降低0.94%(p>0.05)。结果表明单一益生菌及混合益生菌干预可通过改善肠道SCFAs的含量,有助于调节肠道环境稳定,且以混合菌株的作用效果更佳。
[Key word]
[Abstract]
To explore the effect of probiotics intervention on the short-chain fatty acid content in feces of hyperlipidemic rats, the hyperlipidemia rat model was established, and the experimentally preserved Lactobacillus plantarum 67, Lactobacillus fermentum 83 and Lactobacillus rhamnosus LGG with good cholesterol-lowering effect were used, combined with "5:2 light fasting law".The model rats were subjected to probiotic intervention, five times a week, and the content of SCFAs in the feces of rats was determined after 6 weeks of intervention. Compared with the model group, the contents of butyric acid and valeric acid increased by 37.97% and 41.22% (p<0.05) respectively, the content of propionic acid increased by 1.25% (p>0.05), the content of acetic acid reduced by 3.19% (p<0.05) and the contents of isobutyric acid and isovaleric acid were respectively reduced by 2.11% and 14.16% (p>0.05) after the intervention of Lactobacillus plantarum 67. After intervention with mixed strains, the contents of propionic acid, butyric acid, valeric acid and isobutyric acid increased by 31.25%, 70.92%, 103.98% and 24.48%, respectively (p>0.05), the content of acetic acid reduced by 23.38% (p<0.05), and the content of isovaleric acid decreased by 7.38% (p>0.05). Compared with the positive control group, Lactobacillus plantarum 67 significantly increased the contents of acetic acid,, isobutyric acid and isovaleric acid, which were increased by 31.69%, 40.33% and 82.83%, respectively (p<0.05), the content of valeric acid increased by 12.74% (p>0.05), the content of propionic acid decreased by 23.58% (p<0.05), the content of butyric acid decreased by 2.38%(p>0.05). The mixed strain significantly increased the contents of acetic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid, which increased by 4.23%, 20.37%, 78.44%, 62.84% and 109.48%, respectively (p<0.05), and the propionic acid content decreased by 0.94% (p>0.05). Single probiotics and mixed probiotics can improve the level of intestinal SCFAs, help to regulate the intestinal environment, and the effect of mixed strains is better.
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[基金项目]
国家自然科学基金项目(31571855);江苏省高校自然科学研究重大项目(17KJA550004)