本文研究了亚硒酸钠对海水培养蛋白核小球藻的生长和有机硒转化能力的影响，以及富硒蛋白核小球藻中的主要硒形态。通过分批等量添加2 μg/mL至50 μg/mL的亚硒酸钠，确定最佳富硒培养浓度，并采用高效液相色谱法检测藻体中亚硒酸钠、硒代半胱氨酸和硒代蛋氨酸三种主要硒形态的含量。结果表明：亚硒酸钠浓度不宜过高，否则蛋白核小球藻的生长受到抑制。硒浓度为2 μg/mL时小球藻的生物量较高，有机硒含量达到301.40 μg/g，占总硒含量的83.24%；高效液相色谱分析表明在该培养条件下富硒小球藻中有机硒的主要形态为硒代半胱氨酸和硒代蛋氨酸；在测定的三种主要硒形态中未转化的亚硒酸钠仅占23.02%。上述结果说明在海水培养下，小球藻富硒培养的适宜外加硒浓度为2 μg/mL，此条件下长势良好，无机硒得到有效转化，有机硒含量较高，可达到富硒要求。

In this paper, the effects of sodium selenite on the growth of marine cultured Chlorella pyrenoidosa and the transformation ability of organic selenium, as well as the main selenium form in the selenoprotein-rich Chlorella were studied. The optimal selenium-enriched culture concentration was determined by adding 2 μg/mL to 50 μg/mL sodium selenite in batches, and the content of three main selenium forms in algae was determined by HPLC: sodium selenite, selenocysteine and selenomethionine. The results showed that the concentration of sodium selenite should not be too high, otherwise the growth of Chlorella pyrenoidosa was inhibited. When the concentration of selenium was 2 μg/mL, the biomass of Chlorella was high, and the content of organic selenium reached 301.40 μg/g, accounting for 83.24% of the total selenium content. HPLC analysis showed that the main forms of organic selenium in Selenium-enriched Chlorella were selenocysteine and selenomethionine under the culture conditions, and the unconverted sodium selenite accounted for only 23.02% of the three major selenium forms determined. The above results indicate that under seawater culture, the suitable selenium concentration for selenium-enriched culture of Chlorella is 2 μg/mL. Under these conditions, the Chlorella pyrenoidosa grows well; the inorganic selenium is effectively converted, and the content of organic selenium is high, which can meet the requirement of selenium enrichment.

广西创新驱动发展重大专项（桂科AA17202010-3；桂科AA17204075）