[关键词]
[摘要]
本文建立了复方山楂中绿原酸和橙皮苷的含量测定方法,并对比了复方山楂水提物和醇提物的有效成分含量和促消化效果。采用高效液相色谱法测定复方山楂水提物和醇提物中绿原酸和橙皮苷的含量,并进行综合评分;通过保健食品功效学研究中的急性毒性、小鼠小肠运动、大鼠摄食量、食物利用率、胃蛋白酶测定等实验对比复方山楂水提物和醇提物的促消化效果。结果表明,复方山楂水提物有效成分含量综合评分(93.78)高于醇提物(80.04);水提物和醇提物对大鼠、小鼠均无急性毒性,均能显著增加大鼠摄食量(p<0.05);水提物的中、高剂量组及醇提物的高剂量组小鼠小肠推进率提高了26.88%、28.00%、26.55%(p<0.05、p<0.01、p<0.05),大鼠胃蛋白酶排出量增加了38.59%、53.29%、36.64%(p<0.05、p<0.01、p<0.05),醇提物的其他剂量组小肠推进率和大鼠胃蛋白酶排出量无显著性差异(p≥0.05);水提物和醇提物的中、高剂量组大鼠胃蛋白酶活性分别增强了75.96%、125.07%、70.38%、109.38%(p<0.05、p<0.01、p<0.05、p<0.01)。复方山楂水提物有效成分含量和促消化效果优于醇提物。
[Key word]
[Abstract]
A method for the determination of chlorogenic acid and hesperidin in Fufang Shanzha was established in this work. And compare the content of active ingredients in Fufang Shanzha prepared by different solvents (ethanol and water) and their digestive promotion effect was investigated. The contents of chlorogenic acid and hesperidin were determined by HPLC. The comprehensive score was carried out based on their content and paste-forming rate. According to the principles offunctional food, the acute toxicity, the distance of ink movement in small intestine in mice, the body weight, food intakes, food efficiency ratio and the secretion amount of gastric juice and pepsin activity were evaluated. The results showed that the comprehensive score of active ingredients of water extraction (93.78) was higher than ethanol extraction (80.04). Water extraction and ethanol extraction had no acute toxicity to mice and rats. All of them could significantly increase the food intake of rats (p<0.05). The small intestine movement rates in middle dosage, high dosage group of water extraction and high dosage group of ethanol extraction increased by 26.88%, 28.00%, 26.55% (p<0.05, p<0.01, p<0.05), respectively. In addition, the secretion of pepsin in rats in middle dosage, high dosage group of water extraction and high dosage group of ethanol extraction increased by 38.59%, 53.29%, 36.64% (p<0.05, p<0.01, p<0.05), respectively. However, there was no significant difference in small intestinal movement rates and pepsin excretion of rats in other dose groups of ethanol extraction (p≥0.05). The activity of pepsin increased by 75.96%, 125.07%, 70.38%, 109.38% (p<0.05, p<0.01, p<0.05, p<0.01) in the middle dosage, high dosage group of water extraction and ethanol extraction, respectively. For the content of active ingredients and the digestive promotion effect, the water extract from Fufang Shanzha was superior to the ethanol extract.
[中图分类号]
[基金项目]
四川省宜宾县人民政府-西南医科大学科技专项项目[2017-YBXNYD-3(8/13)];四川省叙州区人民政府-西南医科大学科技专项项目(2019-YBXNYD-4);西南医科大学大学生创新创业训练计划项目(2019465)