本文研究了抗坏血酸葡萄糖苷（2-O-α-D-glucopyranosyl-L-ascorbic acid，简称AA-2G）/β-环糊精包合物的制备工艺，以提高它在应用中的稳定性、生物利用度。选用β-环糊精（β-cyclodextrin，β-CD）对AA-2G进行包合，采用饱和水溶液法研究了AA-2G-β-CD包合物的制备工艺。以包合率为考察指标，通过单因素试验考察了温度、时间、搅拌速度以及β-环糊精和AA-2G的摩尔比对包合物制备效果的影响。进一步运用正交试验研究确定了AA-2G-β-CD包合物的最佳工艺条件为：AA-2G与β-CD的摩尔比为1:3，温度为60 ℃、搅拌速度为200 r/min，时间为5 h时，包合率为49.55%。影响包合率的因素顺序为：时间>温度>转速>摩尔比。验证试验表明，饱和水溶液法制备AA-2G-β-CD包合物工艺稳定。通过傅里叶红外色谱法对制备的AA-2G-β-CD包合物进行了鉴定，证明了AA-2G-β-CD包合物的形成。通过抗氧化性实验发现，包合物清除氧自由基能力高于AA-2G与β-CD混合物。综上，采用饱和水溶液法制备AA-2G-β-CD包合物，经验证AA-2G-β-CD包合物形成，通过正交实验优化制备工艺后，其包合率达到49.55%，同时包合物的抗氧化性能力高于AA-2G与β-CD混合物。

In order to improve the stability and bioavailability of 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), the inclusion compound of AA-2G in beta-cyclodextrin (β-CD) was prepared. The preparation process of AA-2G-β-CD inclusion compound was studied by saturated aqueous solution method. The effects of temperature, time, stirring speed and the molar ratio of β-CD to AA-2G on the preparation of inclusion compound were investigated by single factor experiments. The optimized conditions for the preparation of AA-2G-β-CD inclusion compound were determined by orthogonal experiments listed as follows: the molar ratio of AA-2G to β-CD as 1:3, temperature as 60 °C, the stirring speed as 200 r/min, time as 5 h, and the resulting inclusion rate was 49.55%. The order of factors affecting inclusion ratio was time>temperature>speed>molar ratio. The validation test showed that the preparation of AA-2G-β-CD inclusion compound by saturated aqueous solution method was stable. The AA-2G-β-CD inclusion compound was identified by FT-IR, proving the formation of AA-2G-β-CD inclusion compound. It was found that the free radical scavenging ability of the inclusion compound was higher than that of the AA-2G and β-CD mixture. In summary, AA-2G inclusion compound was prepared by saturated aqueous solution method. The formation of AA-2G-β-CD inclusion compound was confirmed. The inclusion rate of AA-2G-β-CD inclusion compound reached 49.55% after optimizing the preparation process by orthogonal experiments. At the same time, the antioxidant ability of the inclusion compound was higher than that of the AA-2G and β-CD mixture.

国家自然科学基金资助项目(21878155)；江苏省先进生物制造协同创新中心项目；江苏省品牌专业建设工程项目；江苏省优势学科建设工程项目，江苏高校“青蓝工程”计划项目；江苏省“六大人才高峰”高层次人才计划项目