本文研究了蓝莓花青素对老龄大鼠肝组织抗氧化功能及凋亡相关基因的影响。实验以SD老龄大鼠为模型，连续灌胃不同剂量的蓝莓提取物30 d后采尾血测定超氧化物歧化酶（SOD）活力、丙二醛（MDA）、还原型谷胱甘肽（GSH）和蛋白质羰基含量；取肝脏，显微镜下观察肝组织病理形态学变化，测定肝脏组织中抗氧化和凋亡相关基因表达水平。结果表明，蓝莓提取物干预后能显著降低老龄大鼠机体MDA和蛋白质羰基含量至7.81±1.55 nmol/mL和0.92±0.42 nmol/(mg prot)，SOD活力增加至134.61±12.27 U/mL；光镜结果显示蓝莓提取物各组肝脏形态好于老龄对照组。且与老龄对照组比较，蓝莓提取物组老龄大鼠肝脏组织中抗氧化基因表达水平上调（SOD1、GPx1、CAT和Gclm），凋亡基因表达水平下调（Caspase-9和Apaf1）。上述结果表明，蓝莓提取物可改善老龄大鼠机体氧化应激状态，且具有较好的保肝作用，其机制可能与增强抗氧化能力和抑制肝细胞凋亡有关。

To explore the effect of blueberry extract on hepatic oxidative stress and apoptotic effects in aged rats, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH) and protein carbonyl levels in serum of aged rats were determined after treatment with different dose blueberry extract for 30 days. Liver sections were examined by optical microscopy. In addition, the expression of antioxidant gene and apoptosis gene were detected by RT-PCR in liver tissue. The results showed that, MDA and protein carbonyl levels in the high dosage with blueberry extract were significantly reduced to 7.81±1.55 nmol/mL and 0.92±0.42 nmol/(mg prot), and SOD level of blueberry extract supplemented groups was significantly increased to 134.61±12.27 U/mL. The liver sections showed that the blueberry extract could keep the hepatocytes better in morphology. Moreover, the expression of antioxidant genes were significantly up-regulated (SOD1, GPx1, CAT and Gclm) and apoptosis genes were down regulated (Caspase-9 and Apaf1) in blueberry extract treatment groups. The results suggest that blueberry extract could relieve oxidative stress of aged rats and has a good hepatic-protective and anti-aging effect. Its mechanism may be related to promote antioxidant capacity and repress hepatocyte apoptosis.

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