[关键词]
[摘要]
本文以柴达木大肥菇为研究对象,采用热水浸提法提取柴达木大肥菇子实体多糖、发酵液多糖和菌丝体多糖,并将三种多糖按照100 mg/kg的剂量分别灌胃小鼠35 d,灌胃结束后分析小鼠力竭游泳时间及力竭游泳结束后小鼠血液中氰化高铁血红蛋白(hemoglobin,Hb)、血清中尿素氮(blood urea nitrogen,BUN)、乳酸脱氢酶(lactate dehydrogenase,LDH)和乳酸(lactic acid,LA)含量、肝糖原(hepatic glycogen,HG)含量变化的差异性,研究柴达木大肥菇多糖的抗疲劳作用。结果表明,从子实体多糖(polysaccharides from sporocarp,PS)、发酵液多糖(polysaccharides from fermentation broth,PFB)和菌丝体多糖(polysaccharides from mycelium,PM)中均分离得到3个多糖组分。PS组、PFB组和PM组中小鼠的力竭游泳时间分别是空白对照组(29.65±1.92 min)的1.93倍、2.33倍和3.03倍。PS、PFB和PM均能提高小鼠血液中Hb、血清中LDH含量和肝脏中HG含量,降低小鼠血清中BUN和LA含量,且PM的效果最明显,PM组小鼠血液中Hb、血清中LDH、BUN、LA和肝脏中HG含量分别是空白对照组(分别为236.72±15.20 g/L、9.95±0.32 Ku/L、16.28±0.04 mmol/L、17.61±1.29 mg/g)的2.19倍、1.27倍、0.26倍、0.38倍和3.05倍。本研究结果说明柴达木大肥菇PS、PFB和PM具有抗疲劳作用,可以提高小鼠的运动耐力、缓解运动疲劳,且PM的抗疲劳效果优于PS和PFB。
[Key word]
[Abstract]
In order to study the anti-fatigue activity of the polysaccharides from Agaricus bitorquis (Quél.) Sacc.Chaidam, polysaccharides were extracted from the sporocarp, fermentation broth and mycelium of Agaricus bitorquis (Quél.) Sacc.Chaidam by hot water, respectively. Then mice were treated by intragastric administration with three kinds of polysaccharides at the dose of 100 mg/kg for 35 days. After last treatment, mice were forced to swim, and the forced swimming time and the changes of hemoglobin (Hb) level in mice blood, the blood urea nitrogen (BUN), lactate dehydrogenase (LDH) and lactic acid (LA) levels in mice plasma and the hepatic glycogen (HG) level were recorded. The results showed that there were three kinds of fractions in the polysaccharides sporocarp (PS), polysaccharides from fermentation broth (PFB) and polysaccharides from mycelium (PM). In the forced swimming test of mice, the swimming time of mice in PS group, PFB group and PM group were 1.93, 2.33 and 3.03 times higher than that of the control group (29.65±1.92 min), respectively, and the PS, PFB and PM not only could increase the Hb level in mice blood, HG level in mice liver and LDH level in mice plasma, but also could decrease the BUL and LA levels in mice plasma. The PM showed better effects than PS and PFB, and the levels of Hb in mice in mice blood, LDH, BUN, LA in mice plasma and HG in PM group were 2.19, 1.27, 0.26, 0.38 and 3.05 times higher, respectively, than that of control group (236.72±15.20 g/L, 9.95±0.32 Ku/L, 16.28±0.04 mmol/L, 17.61±1.29 mg/g, respectively). These results indicated that the PS, PFB and PM from Agaricus bitorquis (Quél.) Sacc.Chaidam performed the anti-fatigue activity, improving the exercise endurance and reliving fatigue in exercise, and that the PM showed better anti-fatigue activity than PS and PFB.
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[基金项目]
国家自然科学基金地区项目(31460391);青海省国际合作项目(2018-HZ-801)