本文采用中波紫外照射的人皮肤成纤维细胞(HSF)和人永生化皮肤角质形成细胞(HaCaT)细胞衰老模型，探索了红景天的4种萃取物—红景天总皂苷、总黄酮、多糖和挥发油的体外抗皮肤衰老作用。结果显示所试的4种萃取物都具有与浓度相依的抗衰老作用，其效果顺序依次为皂苷＞多糖＞黄酮＞挥发油。其中，浓度为800 μg/mL的皂苷的抗衰老效果是维生素C对照组的2倍。黄酮和多糖对HSF细胞具有相当的修复效果，且都好于挥发性油；在HaCaT细胞的场合多糖、黄酮和挥发油具有相类似的抗衰老效果。可见红景天的不同粗提物对人皮肤细胞有一定的抗衰老修复能力，这为深入地研发红景天抗衰老、抗辐射产品提供一定的理论依据。

The anti-aging effect of four extracts (saponins, flavonoids, polysaccharides and volatile oils) from Rhodiola was investigated using the anti-aging model with damaged human skin fibroblasts (HSF) and (Human immortalized keratinocytes) HaCaT cells irradiated by medium-wavelength UV. The results showed that the test four extracts exhibited a concentration-dependent anti-aging effect on HSF and HaCaT cells, with the decreasing activity in the order: saponins > polysaccharides > flavonoids > volatile oils. The anti-aging activity of saponins at 800 μg/mL was 2 times that of the control (Vc). Flavonoids and polysaccharides have the similar repairing effects on demaged HSF cell, which were better than volatile oils. In the case of the HaCaT cell, polysaccharides, flavonoids and volatile oils had the same anti-aging effects. Therefore, it could be concluded that different extracts of Rhodiola have an anti-aging ability to repair demaged human skin cell, which may provide a theoretical basis for the development of Rhodiola products with anti-aging and anti-radiation activities.

广州市科技计划项目（201604020150）