报道了海带内生真菌白地霉Galactomyces geotrichum次生代谢产物的乙酸乙酯萃取物具有改善小鼠学习、记忆能力的作用。以D-半乳糖所致的阿尔兹海默病（AD）小鼠为模型，采用Morris水迷宫实验测定了不同剂量组的上述萃取物对小鼠血清、肝脏和脑部组织中的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性和丙二醛（MDA）的含量及脑组织中乙酰胆碱转移酶（ChAT）和乙酰胆碱酯酶（AchE）的活性的影响。Morris水迷宫结果显示，试验各剂量组的逃避潜伏期的时间均显著缩短（p<0.05），而空间探索能力也有所改善；与模型组相比，低、中、高剂量组血清、肝脏与脑部组织的SOD、GSH-Px、CAT活力升高，而MDA含量降低，脑部组织中AchE活力下降、ChAT活力上升。上述发现说明，该次代谢产物的乙酸乙酯萃取物对AD模型小鼠具有明显改善学习记忆能力的作用。

This work reported that the ethyl acetate extract of secondary metabolites of endophytic fungus Galactomyces geotrichum could improve the memory and learning ability of mice. With D-galactose-induced the Alzheimer's disease (AD) mice as the model, the influence of different levels of dosages of the extract on the contents of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) and malondialdehyde (MDA) in serum, liver and brain of the mice was investigated, using the Morris water maze test. The activities of choline acetyltransferase (ChAT) and acetylcholinesterase (AchE) in mice brain were also evaluated. The results showed that the escape latent period of the mice in each dose group was significantly shortened(p<0.05), and there was also noticable improvement in the spatial probe capability of the mice. Compared with the model group, the activities of SOD, GSH-Px and CAT in serum, liver and brain tissue of the low, middle and high dose groups were increased, while the MDA content decreased, the activity of AchE decreased, as well as that of ChAT increased in brain tissue. The findings indicated that the ethyl acetate extract of secondary metabolites could improve the memory and learning ability of the AD mice.

山东省自然科学基金项目（ZR2015BM016）