本文通过研究菌落总数测试片中无纺布的种类，加工工艺，冷水可溶性凝胶与培养基配比及质构特性，应用于需氧菌计数测试片，对其检测性能进行评价。对菌落总数测试片中冷水可溶性凝胶与培养基在不同比例配比情况下质构特性进行测定，当凝胶与培养基比例为3:7时，硬度、脆性、粘着性、弹性、内聚性、胶着性、回弹性与1%琼脂培养基无显著性差异，并选取60 g/m2的水刺无纺布为载体，采用浸入培养基加入方式，菌落生长情况良好，清晰可见，菌落计数方便。基于本研究以无纺布为载体测定5种试验菌株的菌落总数并与国标需氧菌平板计数法比较，可达到相同水平的检测限2 CFU/mL和灵敏度（100%）；准确度较高，线性相关系数R2达到0.996。水刺无纺布可作为菌落总数测试片的良好载体。

The performance of non-woven fabric in Petrifilm Aerobic Count Plates was evaluated by investigating the types of non-woven fabric, processing technology, theratio of cold water soluble gel and medium and texture characteristics. When the ratio of gel to medium was 3:7, the hardness, fracturability, adhesiveness, cohesiveness, springiness and gumminess were similar with those of 1% agar medium, and the 60 g/m2 of spunlace non-woven fabric was selected as the carrier . The petrifilm plate method based on prepared cold water soluble gel could achieve the same limit of detection (2 CFU/mL) and sensitivity (100%) in colony counting of five testing strains compared with national standard aerobic bacteria plate counting method. The petrifilm plate method based on cold water soluble gel had high accuracy and linearly dependent coefficient (R2=0.996). These two methods have no significant difference (p>0.05). The spunlaced non-woven fabric can be used as a good carrier of petrifilm aerobic count plate.

吉林省科技发展计划资助项目（20180201048NY）