本文探究了胶球藻C-169的营养方式，并在未灭菌的脱色酵母废水中高密度接种胶球藻C-169，系统比较了接种密度、废水浓度对胶球藻C-169的生长及其处理酵母废水效果的影响。结果表明，胶球藻C-169可以利用葡萄糖和蔗糖进行异养和混养生长。混养条件是胶球藻C-169快速扩种的最佳条件。在混养条件下，胶球藻生长速度最快，最适葡萄糖浓度为20 g/L。通过单因素实验研究培养条件（接种密度、废水起始浓度）对胶球藻C-169的生长和废水处理效果的影响，建立的最佳培养条件为：起始接种密度不低于1 g/L，脱色废水稀释倍数为2倍。在此条件下，胶球藻C-169的生物量产率为0.23 g/(L·d)，脱色废水COD（化学需氧量）、总氮、总磷的去除率分别为49.54%、70.39%和98.09%。此研究表明胶球藻C-169在藻菌共生条件下对酵母废水的净化具有很大潜力。

This paper investigated the optimal parameters for high-density inoculation of Coccomyxa subellipsoidea C-169 under different nutritional conditions in yeast fermentation wastewater. The effects of inoculation density and wastewater concentration on the growth of C-169 and the treatment of yeast wastewater were systematically compared. Results showed that C-169 could use glucose and sucrose for heterotrophic and mixotrophic cultivation. Under cultivation conditions, the growth rate of C-169 was the fastest and the optimal glucose concentration was 20 g/L. The effects of culture conditions (inoculation density and initial wastewater concentration) on the growth of C-169 and the treatment of yeast wastewater were studied by single-factor experiments , and the optimal culture conditions were as follows: the initial inoculum density was not less than 1 g/L, and the dilution rate of decolorization wastewater was 2 times. Under this condition, the biomass yield of C-169 was 0.23 g/(L·d), and the removal rate of COD (chemical ocygen demand), total nitrogen and total phosphorus in decolorization wastewater were 49.54%, 70.39%, 98.09%, respectively. Consequently, this study illustrated that C-169 had great potential for the purification of yeast wastewater under algal-bacterial coexistence conditions.

广州市科技计划项目（201704030084）；国家863计划项目（2013AA065802）；广东省海洋渔业科技与产业发展专项（A201401C01）；广东省公益研究与能力建设项目（2015A020216003、2016A010105001）