[关键词]
[摘要]
本文对从冰冻海鱼中分离到的副溶血弧菌菌株229进行基因组测序,并对注释的基因进行功能聚类分析,以分析其致病因子,为研究其致病机制提供基因组全局水平的数据支持。完成了副溶血弧菌菌株229的基因组测序及组装,所得基因组4.97 Mb,GC含量为43.90%。基因注释获得4940个蛋白质编码基因(CDS),其中1304个CDS具有GO功能注释。WEGO注释显示大量CDS对于副溶血弧菌入侵宿主及随后的代谢过程发挥重要作用。获得了与副溶血弧菌致病性相关的致病因子,包括溶血素(hemolysin)、Ⅲ型因子(Type Ⅲ secretion system,TTSS),这些因子位于不同的染色体位点,形成独立的致病因子簇。副溶血弧菌菌株229基因组组装和功能注释为研究副溶血弧菌致病性的遗传学基础提供了有价值的数据。
[Key word]
[Abstract]
In this study, vibrio parahaemolyticusisolate 229 isolated from frozen sea fish was carried out by genome sequencing, and gene annotation was performed by gene functional analysis to elucidate its pathogenic factors, which could provide clues and genome-scale data for investigating its pathogenic mechanism. We have accomplished the genome sequencing of V. parahaemolyticus 229 and assembled its genome using the obtained paired-end sequencing reads generated on the Illumina Hiseq 2000 Genome Analyzer. The assembled genome was 4.97 Mb in size with GC content of 43.90%. 4,940 protein coding sequences (CDSs) were predicted by gene prediction, of which 1,304 CDSs were annotated with GO function. WEGO illustration revealed that a series of CDSs might play important roles in theinvasion of the host of V. parahaemolyticus and subsequent metabolic processes. And an array of pathogenic virulence factor genes were annotated including hemolysin and Type III secretion system, which were gathered in different contigs and might act as independent pathogenic factors. The assembled genome and functional annotation of V. parahaemolyticus 229 could provide valuable information for further investigating the genetic basis of its pathogenicity.
[中图分类号]
[基金项目]
广东省科技计划项目(2016A050503016、2016A010105004);华南理工大学中央高校基本科研业务费资助项目(2015ZP032);广东省自然科学基金项目(2017A030313097、S2012030006235)