[关键词]
[摘要]
以猕猴桃为研究对象,测定对照组和不同膨大处理(CPPU、TDZ)的猕猴桃总酚、以及总还原能力、羟基自由基清除率、超氧自由基清除率和ABTS自由基清除率,分析不同膨大处理对总酚和抗氧化活性的影响,筛选出影响较大的膨大处理组;并研究其不同残留量对总酚含量和抗氧化活性的影响,作为膨大剂允许残留量影响抗氧化活性的参考。结果表明:膨大处理TDZ显著降低总酚含量和抗氧化活性,对总还原能力、羟基自由基清除率、超氧自由基清除率的影响较大,对ABTS自由基清除率的影响较小。研究不同TDZ残留量的影响,表明残留量为0.04 mg/kg可显著降低总酚含量,TDZ残留量大于0.02 mg/kg时即可显著降低总还原能力和羟基自由基清除能力,TDZ残留量大于0.04 mg/kg才显著降低对ABTS自由基和超氧自由基的清除能力。TDZ对总还原能力和羟基自由基清除能力的影响较大,对ABTS自由基和超氧自由基的清除能力影响较小。
[Key word]
[Abstract]
The kiwifruit was used as the research object to determine the total phenols, total reducing ability, hydroxyl free radical scavenging rate, superoxide free radical scavenging rate and 2,2‘-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging rate of the kiwifruit in the control group and different swelling agent treatment groups (CPPU and TDZ). The effect of different swelling agent treatments (CPPU and TDZ) on the total phenols and antioxidant activity of Kiwifruit were determined forand screening out for their possible impact. The result showed that total polyphenols content and antioxidant activity of Kiwifruit could be reduced by TDZ treatment. In brief, the TDZ treatment had a significant effect on the total polyphenols content, total reducing capacity, hydroxyl radical scavenging rate and superoxide radical scavenging rate, had little effect on the ABTS+?scavenging rate. The result showed that the content of polyphenols was significantly reduced at the TDZ residual concentration of 0.04 mg/kg. The residual of TDZ more than 0.02 mg/kg could significantly reduce the total reducing power and hydroxyl radical scavenging capacity. The residual of TDZ more than 0.04 mg/kg could significantly reduce the ABTS+? scavenging capacity and superoxide radical scavenging capacity. And TDZ had a greater influence on the total reducing power and hydroxyl radical scavenging capacity, and had little effect on the ABTS+?scavenging capacity and superoxide radical scavenging capacity.
[中图分类号]
[基金项目]
中央高校基本科研业务费专项基金资助(2662016PY100)