利用离子液体([Bmim]BF4)双水相提取香樟叶中的黄酮，首先确定稳定的双水相体系，在单因素试验基础上，选择浸提时间、浸提温度、溶液pH为影响因子，应用Box-Benhnken中心组合法进行3因素3水平试验设计，以香樟叶总黄酮得率为响应值，进行响应面分析，利用Al(NO3)3-NaNO2-NaOH分光光度法测定黄酮的含量。研究结果表明：稳定的离子液体双水相体系：成相盐为硫酸铵，加入量为0.8 g，[Bmim]BF4与水的体积比为2:2；响应面分析法的优化提取条件：0.1 g的香樟叶粉末时，得出对黄酮提取率影响大小的因素依次为提取时间、提取温度、pH，最佳浸提条件为：浸提时间3.70 h，浸提温度82 ℃，pH为9.60，在此条件下黄酮提取率达10.62%。同时建立了离子液体双水相提取香樟叶叶总黄酮的二次数学模型，对目标产物提取具有良好的预测作用，为该体系的放大实验或规模化生产提供理论指导。

Flavonoids of camphor leaves was extracted by an ionic liquid/aqueous two-phase system in this paper. First, the stability of aqueous two-phase was investigated. Based on single-factor tests, Box-Benhnken center composite experiment was carried out with three factors and three levels of experimental design using extraction time, extraction temperature and pH as the influence factors. Besides, the content of flavonoids was determined by Al(NO3)3-NaNO2-NaOH spectrophotometric method. For a stable ionic liquid aqueous two-phase system, the phase-forming salt is ammonium sulfate ( (NH4)2SO4); the addition amount is 0.8 g; and the volume ratio of the [Bmim] BF4 to water was 2: 2. Response surface analysis showed that for 0.1 g of camphor leaves powder, the influence of factors that affected the extraction rate of flavonoids were in a descending order of extraction time, extraction temperature and pH. The optimized extraction conditions were as follows: extraction time 3.7 h, extraction temperature 82℃, and the solution acidity 9.6. Under the optimum conditions, the extraction rate of flavonoids reached 10.62%. At the same time, the secondary mathematical model for extraction of total flavonoids from camphor leaves with ionic liquid aqueous two-phase system was established, which has a good predictive effect on target product extraction, and provided theoretical guidance for the scale-up experiment or large-scale production of this system.

国家自然科学基金青年基金项目（51704042）；常州工学院科研基金项目（YN1210）