本文研究了千日红粗提物对酪氨酸酶的抑制作用并采用响应面法优化千日红中酪氨酸酶抑制剂的提取工艺。在单因素实验的基础上，探讨了提取温度、提取时间、液料比和乙醇浓度等因素为影响因子，应用Box-Benhnken中心组合法进行4因素3水平试验设计，以酪氨酸酶抑制率率为响应值，进行响应面分析，优化千日红中酪氨酸酶活性抑制组分的提取工艺。结果表明，最佳提取条件：提取温度为80 ℃、提取时间为3 h、液料比为60 mL/g(V/M)、乙醇浓度为50%(V/V)。在此条件下，RSM模型预测千日红乙醇粗提物对酪氨酸酶的抑制率为54.94%，实测千日红乙醇粗提物对酪氨酸酶的抑制率为（53.86±2.11）%，与预测值无显著性差异(p>0.05)。

Inhibitory effect of Gomphrena globose (GG) crude extract on the tyrosinase activity was investigated and the extraction process of tyrosinase inhibitor was optimized by response surface methodology (RSM). Based on single-factor tests, the extraction temperature, extraction time, liquid-to-solid ratio and ethanol concentration were regarded as factors. A 4-factor, 3-level experiment design was performed using the Box-Benhnken center composite experiment. The response surface analysis was conducted by using tyrosinase inhibition rate as the response value, and the extraction process of the inhibitory component of tyrosinase activity was optimized.The results showed that the optimum extraction conditions by RSM were extraction temperature of 80℃, extracting time of 3 h, ratio of liquid to material of 60:1 (V/M) and the concentration of ethanol 50% (V/V). Under the conditions, the RSM model predicted that the inhibition rate of crude extract on tyrosinase was 54.94%, which was no significant difference compared with the actual measured value (53.86 ± 2.11)% (p > 0.05).

深圳职业技术学院科研项目（601722K20024）