[关键词]
[摘要]
为了评价甜菜碱的抗氧化活性,本文研究了甜菜碱对受AAPH诱导产生的自由基损伤的红细胞和肝细胞的影响。结果显示当甜菜碱的浓度达到200 mmol/L时,AAPH诱导的红细胞氧化溶血率降低了54.19%,MDA降低了4.32 nmol/mg;电镜下阳性损伤组红细胞胞膜皱缩明显、棘突较多、呈星状改变,而保护组胞膜只是略有皱缩,恢复至阴性组正常细胞形态;同时经甜菜碱处理,阳性损伤组红细胞胞内ROS含量降低了57.16 IU/mg,SOD、CAT、GPx酶活分别降低了9.84 mU/mg、13.91 mU/mg和43.59 mU/mg,并与阴性对照组无显著性差异;在正常肝细胞中,50 mmol/L甜菜碱使AAPH损伤的细胞内MDA下降了0.21 nmol/mg,SOD、CAT酶活则分别下降了173.21 U/mg和53.9U/mg,与阴性组正常组肝细胞无显著性差异。综上分析,甜菜碱能很好的保护细胞免受AAPH产生的自由基的损伤,说明甜菜碱具有良好的抗氧化作用。
[Key word]
[Abstract]
The antioxidant activities of betaine were evaluated by cellular antioxidant activity assay, AAPH-induced erythrocyte assay, and AAPH-induced liver cell assay. The results revealed that betaine (200 mmol/L) treatment effectively reduced AAPH-induced erythrocyte hemolysis by 54.19%, and the MDA content by 4.32 nmol/mg. Erythrocytes induced by AAPH was appeared shrinking under scanning electron microscope, however, erythrocytes prior treated with betaine was only presented slight shrinking. Moreover, after beanie treatment, the amount of ROS in AAPH-induced erythrocytes declined by 57.16 IU/mg, and the activities of SOD, CAT, and GPx decreased by 9.84 mU/mg, 13.91 mU/mg, and 43.59 mU/mg, which were same as the normal levels. The betaine (50 mmol/L) treated in liver cell after AAPH-induced, MDA level was dropped by 0.21 nmol/mg and the SOD and CAT activities decreased by 173.21 U/mg and 53.9 U/mg, which had no significant difference with the normal cell. In conclusion, betaine could protect cells from the damage of radical induced by AAPH, showing that betaine has good antioxidant activities.
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[基金项目]
国家自然科学基金项目(31201330);广州市科技攻关项目(201300000202)