以实验室选育的一株侧孢短芽孢杆菌Brevibacillus laterosporus S62-9为对象，体外研究其耐热性、耐酸性、耐胆盐性、黏附性以及药敏性等生物学特性。结果表明，B. laterosporus S62-9耐热性较好，85 ℃水浴5 min，存活率达86.20%；人工胃液中作用4 h，pH 2.0下的存活率最低，为85.70%，pH 3.0和pH 4.0下的存活率均高于95.00%；人工肠液中作用4 h，胆盐浓度为0.1%、0.2%和0.3%时的存活率分别为93.30%，91.70%和88.30%；对模拟胃肠道消化过程的耐受性高，pH 2.0胃液处理2 h后又经0.3%胆盐处理3 h，存活率达96.00%；营养体状态的B. laterosporus S62-9对Caco-2细胞体外的粘附率为20.70 CFU/cells，黏附能力高于对照组枯草芽孢杆菌K1；对养殖中常用的10种抗生素均未产生耐药性，是安全菌株。B. laterosporus S62-9优良的体外益生菌特性符合微生态制剂菌株的选育标准和要求，为其微生态制剂的开发与应用奠定了理论基础。

The thermal resistance, acid resistance, bile salt resistance, adhesion ability, antibiotic susceptibility, and other biological characteristics of Brevibacillus laterosporus strain S62-9 were studied in vitro. Strain S62-9 exhibited good thermal tolerance, with a survival rate of 86.20% after incubation in an 85 ℃ water bath for 5 min. It had the lowest survival rate (85.70%) after exposure to simulated gastric juices at pH 2.0 for 4 h, whereas the survival rates at pH 3.0 and 4.0 were both higher than 95.00%. The survival rates after 4-h treatments in 0.1%, 0.2%, and 0.3% bile-salt-stimulated intestinal fluids were 93.30%, 91.70%, and 88.30%, respectively. Strain S62-9 showed high tolerance to simulated gastrointestinal transit, where its survival rate was 96.00% after being treated with gastric juice at pH 2.0 for two hours followed by a three-hour treatment in intestinal fluid containing 0.3% bile salt. The bacterium in the trophozoite stage showed an adhesion ability of 20.70 CFU per Caco-2 cell, which was higher than that of the control strain (Bacillus subtilis K1). Strain S62-9 had no observable resistance to 10 types of antibiotics tested and was thus considered to be a biosafe strain. These excellent probiotic characteristics of B. laterosporus S62-9 meet the standards and requirements of a good probiotic, laying a theoretical basis for its development and application as a probiotic strain.

国家科技支撑计划（2013BAD10B03）；国家农业科技成果转化项目（2013GB2A200032）；河北省自然科学基金（C2014208137）