本文在前期试验的基础上，获得了树莓、红豆越橘、蓝靛果、猕猴桃、山楂和山荆子6种多酚以及黑木耳碱性多糖提取物，检测单一组分和复配物(浆果多酚与黑木耳多糖质量比1:1)对羟基自由基、ABTS+?和DPPH?的清除能力和总还原能力。通过Chou-Talalay联合指数(CI)，分析它们是否具有协同抗氧化作用。树莓与黑木耳多糖的复配物对羟基自由基和DPPH?清除率达50%时，CI羟基自由基和CIDPPH分别为0.56±0.09和0.45±0.19，表明复配后可以提高对这两种自由基的清除效率，起到协同抗氧化作用。猕猴桃与黑木耳多糖的复配物对ABTS清除率和总还原力达50%时，CIABTS和CI总还原力分别为0.48±0.11和0.42±0.05，揭示其复配物的抗氧化能力明显增强。深入研究多成分的协同作用将更有利于开发新的、安全和高效的天然抗氧化保健品和相关药物，为植物功能性成分的开发利用提供理论依据。

Six types of polyphenols extracts were obtained from raspberry, lingonberry, blue honeysuckle, kiwi fruit, hawthorn, and Siberian apple, as well as an Auricularia auricular polysaccharide（AAP）extract. The scavenging capacities of the hydroxyl radicals, [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS+?) and 2,2-diphenyl-1-picrylhydrazyl (DPPH?), as well as the total reducing capacity of a single and compound extract (mass ratio of berry polyphenols and AAPs = 1:1), was measured. The Chou-Talalay combination index (CI) was used to analyze whether there were synergistic antioxidant effects among these extracts. When the clearance rates of the compound extract of raspberry-AAPs on hydroxyl radicals and DPPH? reached 50%, the values of CIhydroxyl radicals and CIDPPH were 0.56 ± 0.09 and 0.45 ± 0.19, respectively, indicating that compounding could improve the scavenging efficiency of the 2 radicals. There was a synergistic antioxidative effect. When the clearance rate and total reducing power of the compound extract of kiwi fruit-AAPs on ABTS was 50%, the values of CIABTS and CITR were 0.56 ± 0.09 and 0.45 ± 0.19, respectively, suggesting there was an improved antioxidant capacity of this compound extract. Further investigation of the synergistic effects of multi-components is important for the development of new, safe, and highly efficient natural antioxidant health care products and related compounds, providing a theoretical basis for the development and utilization of plant functional ingredients.

国家自然科学基金（31170510）；黑龙江省应用技术研究与开发计划项目（GA13B202）