[关键词]
[摘要]
分析研究金边黄杨叶、茎挥发油的主要成分及其抗肿瘤活性。以超临界二氧化碳萃取金边黄杨叶、茎挥发油,利用气相色谱-质谱联用技术(GC-MS),经过NIST08谱库检索对比,从中分别,叶挥发油鉴定31个化合物,主要成分有:棕榈油酸(17.11%)、苯甲醛(10.66%)、(Z)-3-己烯-1-醇(8.93%)、正十六烷酸(7.92%)、苯甲醇(6.99%)、肉豆蔻酸(6.13%)、正十五烷酸(6.06%)。茎挥发油鉴定33个化合物,主要成分有:丙二醇单甲醚(12.20%)、正十六烷酸(5.52%)、6,6-二甲基二环[3.1.1]庚-2-烯-2-甲醇(5.17%)、2,4-二叔丁基苯酚(5.08%)。应用四甲基偶氮唑盐(MTT)法检测抗癌活性,结果叶挥发油对对肺癌细胞A549和胃癌细胞SGC-7901抑制最强,其IC50值分别为2.77 μg/mL、3.78 μg/mL,对比研究表明正十五烷酸是叶挥发油中抑制两种癌细胞的主要活性成分。
[Key word]
[Abstract]
The chemical composition and anti-tumor activity of the volatile oil from the leaves and stems of the Euonymus japonicus L. f. aureo-marginatus Rehd. were studied. The volatile oils from the leaves and stems of Euonymus japonicus L. f. aureo-marginatus Rehd. were extracted with CO2 as the supercritical solvent. The resulting sample was separated and detected by gas chromatography mass spectrometry (GC-MS). Based on a comparison with the NIST08 mass spectral library, 31 components were identified in the volatile oil from the leaves. The main constituents included: cis-9-hexadecenoic acid (17.11%), benzaldehyde (10.66%), (Z)-3-hexen-1-ol (8.93%), n-hexadecanoic acid (7.92%), benzyl alcohol (6.99%), tetradecanoic acid (6.13%), and n-pentadecanoic acid (6.06%). Thirty-three components were identified from the volatile oil of the stems. The main constituents were 1-methoxy-2-propanol (12.20%), n-hexadecanoic acid (5.52%), 6,6-dimethyl-bicyclo [3.1.1]hept-2-ene-2-methanol (5.17%), and 4-di-tert-butylphenol (5.08%). The tetrazolium salt (MTT) assay was used to measure the anti-tumor activity, and showed that the leaf volatile oil exhibited the strongest anti-tumor activity against A549 lung cancer cells and SGC-7901 gastric carcinoma cells, with IC50 values of 2.77 μg/mL and 3.78 μg/mL, respectively. This comparative study indicates that n-pentadecanoic acid is the major active ingredient of the leaf volatile oil in the 2 types of cancer cells.
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[基金项目]
安徽省质量工程项目(2014zy078,2014jyxm415);安徽新华学院质量工程项目(2014jgkcx14);安徽省大学生创新创业训练项目(AH201412216010)