[关键词]
[摘要]
本研究通过鲨鱼线粒体基因组的部分序列,设计了鲨鱼的特异性引物和探针,建立了鱼翅制品中鲨鱼源性成分检测的一种新方法,据此可鉴别鱼翅制品的品质。通过物种特异性实验和灵敏度测试,表明所设计的引物和探针具有较好的物种特异性,所建立的方法具有较高的灵敏度:DNA浓度的检测灵敏度可达0.01 ng/?L,重量检测灵敏度可达0.1%(m/m)。采用该方法并结合植物源性基因检测方法对市场上随机抽取的25份鱼翅制品进行检测,其中18份样品检出鲨鱼源性成分而未检出植物源性成分,7份样品未检出鲨鱼源性成分而检出植物源性成分,表明这7份样品不是真鱼翅制品,而是采用了植物成分仿制而成。本研究所建立的方法快速、灵敏、简单,适用于市场上鱼翅制品中鲨鱼源性成分的快速检测。
[Key word]
[Abstract]
Specific primers and probes were designed using a portion of the shark mitochondrial genome to establish a novel method for the detection of components of shark-origin in shark fin products, in order to determine product quality. The results of this study indicated that this method had good species specificity, as well as a high sensitivity of 0.01 ng/?L DNA concentration or 0.1% (m/m) weight. This method was then combined with a detection method developed for the identification of plant-origin genes; this combined method was used to examine 25 shark fin products available in the market (randomly chosen). The results of this analysis revealed the presence of shark-origin components (and not plant-origin components) in 18 samples, and of plant-origin components (and not shark-origin components) in the remaining 7 samples; this indicated that the latter 7 samples were imitation fin products prepared using plant ingredients. The method established in this study is fast, sensitive, and simple, and suitable for the rapid detection of shark-origin components in shark fin products available in the market.
[中图分类号]
[基金项目]
国家质检总局科研项目(2013QK278)