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[摘要]
氧化白藜芦醇是一种重要的活性天然产物,是反式二苯乙烯类天然产物白藜芦醇(Resveratrol)的2'-位羟基化衍生物,具有美白、抗癌、保肝、抗氧化、抗衰老、抗炎等作用。本研究在建立了精确检测氧化白藜芦醇高效液相色谱(HPLC)方法基础上,以桑根为原料,研究了氧化白藜芦醇的提取分离、稳态化包埋及对酪氨酸酶活性的抑制作用。结果表明:以80%乙醇为提取溶剂,在提取温度70 ℃,时间1.5 h,液料比4:1,提取次数3次条件下,氧化白藜芦醇提取得率为94.32%;在固形物含量为40%,进风温度为190 ℃,雾化盘转速为16000 r/min,进料速度为1.78 L/h条件下,氧化白藜芦醇微胶囊稳态化包埋率为98.91%;氧化白藜芦醇标准品、氧化白藜芦醇微胶囊样品具有对酪氨酸酶活性抑制作用,且抑制强弱呈剂量依赖型。
[Key word]
[Abstract]
Oxyresveratrol is a 2'-hydroxyl derivative of resveratrol (a trans-stilbene); it is an important natural bioactive product with whitening, anticancer, antioxidant, hepatoprotective, anti-inflammatory, and anti-aging functions. In this study, high-performance liquid chromatography (HPLC) was used to detect oxyresveratrol in mulberry root, and its extraction, separation, steady-state embedding, and inhibitory effects on tyrosinase activity were examined. The extraction rate of oxyresveratrol was 94.32% for the following conditions: 80% ethanol as the extraction solvent, an extraction temperature of 70 ℃, an extraction duration of 1.5 h, a liquid to solid ratio of 4:1, and independent extraction of three times. The steady-state embedding rate of oxyresveratrol was 98.91% for the following conditions: a solid content of 40%, an inlet temperature of 190 ℃, an atomizing disk speed of 16,000 r/min, and a feed speed of 1.78 L/h. The oxyresveratrol standard and oxyresveratrol microcapsule samples both had inhibitory effects on tyrosinase activity, and the strength of inhibition was dose-dependent.
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