[关键词]
[摘要]
研究1-甲基环丙烯(1-MCP)处理对采后‘油木奈’果实衰老的影响及其与能量代谢的关系。采后‘油木奈’果实分别用0(对照)和1.2 μL/L的1-MCP处理12 h,之后在(25±1)℃下贮藏。贮藏期间定期测定果实细胞膜透性,ATP、ADP和AMP含量,NAD激酶(NADK)活性,NAD、NADH、NADP和NADPH含量。结果表明:与对照果实相比,1-MCP处理能延缓‘油木奈’果实细胞膜透性的上升,促进贮藏后期(12~18 d)ATP含量的积累,延缓整个贮藏期间能荷值的下降;1-MCP处理可保持‘油木奈’果实较高的NADK活性,降低NAD和NADH含量,提高NADP和NADPH含量。据此认为,1-MCP处理能较好维持采后‘油木奈’果实细胞膜结构的完整性、延缓果实衰老可能与保持较高的能荷值有关。
[Key word]
[Abstract]
The effect of 1-methylcyclopropene (1-MCP) treatment on the senescence of harvested ‘Younai’ (Prunus salicina Lindl. cv. Younai) plums and its relation to energy metabolism was investigated in this study. Harvested ‘Younai’ plums were treated with 0 (control) and 1.2 μL/L 1-MCP for 12 h, and stored at 25 ± 1 ℃. The cell membrane permeability, ATP, ADP, and AMP content, NAD kinase (NADK) activity, and NAD, NADH, NADP, and NADPH expression was analyzed in the control and sample plums during storage. The results of these analyses revealed that MCP-1 treatment slowed the increase in cell membrane permeability, promoted ATP accumulation during the late stage of fruit storage (12~18 days), and delayed the decrease in the level of energy charge during the whole storage period (compared to the control samples). In addition, 1-MCP treatment resulted in the maintenance of a relatively high NADK activity, a reduction in NAD and NADH content, and an increase in the NADP and NADPH content in the 1-MCP treated-‘Younai’ plums. Based on these results, it was concluded that the MCP-1 treatmentinduced the maintenance of the integrity of the cell membrane structure, and the delay in the senescence of harvested ‘Younai’ plums was correlated with the maintenance of a higher level of energy charge.
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[基金项目]
国家科技支撑计划项目(2007BAD07B06);福建省教育厅科研项目(JA13200);闽南师范大学杰出青年科研人才计划项目(SJ12004);闽南师范大学博士科研启动项目(L21231)