采用基于PCR反应的变性梯度凝胶电泳技术（PCR-denaturing gradient gel electrophoresis，PCR-DGGE），对液熏罗非鱼片在贮藏过程中的菌相组成及变化进行分析，旨在为液熏罗非鱼的贮藏条件提供理论基础。研究结果表明：用DGGE法总共分离到13个条带，测序比对相似度在90%以上的菌种有10种，分别是：2株肠杆菌属（Enterobacter），2株梭菌（Clostridium），2株乳球菌属（Lactococcus），2株戊糖片球菌（Pediococcuspentosaceus），1株明串珠菌（Leuconostoc），以及1株假单胞菌属（Pseudomonas），乳酸菌(LAB)占所有菌株的50%。贮藏早期（<2 d）和末期（>7 d）的菌相较为复杂，可辨识条带>10，贮藏中期（2~7 d）菌相条带表现出集中和明显的特点，可辨识条带仅在5条左右，微生物多样性减少，图谱结合测序分析表明贮藏期的优势菌种为乳酸球菌（Lactococcus）、戊糖片球菌（Pediococcuspentosaceus）和梭菌（Clostridium）。

Denaturing gradient gel electrophoresis technique based on polymerase chain reaction (PCR-DGGE) was used to investigate the constitution and changes of bacterial flora in the liquid-smoked tilapia fillets during storage, in order to provide a theoretical foundation for their storage conditions. The results showed that 13 bands were isolated using DGGE and after analysis their sequences, 10 of them had sequence similarity above 90%. These included two strains of Enterobacter, two strains of Clostridium, two strains of Lactococcus, two strains of Pediococcuspentosaceus, one strain of Leuconostoc, and one strain of Pseudomonas. Lactic acid bacteria (LAB) accounted for 50% of all bacteria. The bacterial flora during early storage (<2 d) and the last storage period (>7 d) were quite complex, with more than 10 identified bands. During the mid-storage period (2~7 d), however, the bacterial DGGE bands were distinctly grouped and there were only approximately five identified bands, indicating that the microbial diversity decreased. The bands from DGGE and sequencing results indicated that the dominant bacteria during storage were Lactococcus, Pediococcuspentosaceusand Clostridium.

国家现代农业产业技术体系项目(CARS-49)；广东省海洋渔业科技推广专项(A201101C01)；2011年度广西教育厅立项项目(201106LX525)