本文从124份市面蜂蜜样品中筛选出21份C-4植物糖超标蜂蜜样品，应用元素分析-同位素质谱联用技术（EA-IRMS）、液相色谱-同位素质谱联用技术（LC-IRMS）、液相色谱测定蜂蜜还原糖含量等检测方法，对C-4植物糖超标蜂蜜样品进行多维分析。结果表明：C-4植物糖超标样品在市面上仍占有一定比例，本批次检出超标率为16.94%，且存在少量严重超标样品，最高达到82.35%。C-4植物糖超标蜂蜜样品大致可归纳为以下三类：一是源于生产操作不规范的轻微超标型，该类样品的碳-4植物糖含量为7~10%之间，△δ13CF-G、△δ13Cmax跟真实蜂蜜要求相差不远；二是人为掺入碳-4植物源淀粉转化产物型，该类样品寡糖含量较高，△δ13Cmax较大。三是人为掺入碳-4植物源果葡糖浆型，该类样品本身不含寡糖，δ13CF、δ13CG完成丧失碳-3植物特征。

In this paper, 21 honey samples with excessive amounts of C-4 plant sugar were chosen from 120 specimens obtained from food market.Element analyzer-isotope ratio mass spectrometry (EA-IRMS), liquid chromatography-isotope ratio mass spectrometry (LC-IRMS) and liquid chromatography (LC) were adopted to detect the δ13C values and the amount of C-4 plant sugar. The results indicated that the honey samples with excessive amounts of C-4 plant sugar still occupy a certain proportion in food market and the over-standard rate of this batch was 16.94%. Several samples were detected with serious excessive C-4 plant sugar, one of which haDs the highest amount of 82.35%. The honey samples with excessive C-4 plant sugar can be summarized as the following three categories. (1) Samples with slightly excessive C-4 plant sugar: the standard-exceeding rate of those samples was 7~10% , and their △δ13CF-G and △δ13Cmax values were not far from the requirements of the real honey. (2) Samples adulterated with starch products of C-4 plant source: these samples had a high amount of oligosaccharide and larger △δ13Cma. (3) Samples adulterated with high fructose corn syrup; no oligosaccharide was found in these samples and their δ13CF and δ13CG completely lost the characteristics of C-3 plant.

广州市科技计划项目(2011J2200016；[2011]233-34)；广东省质量技术监督局科技项目（2011ZS01）