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[摘要]
用间接竞争酶联免疫分析法(ELISA法)对3种玉米油中的玉米赤霉烯酮残留进行了测定,以评价试剂盒的性能。结果表明,标准曲线线性方程y=-2.2x+0.66,相关系数r为0.9974,IC50为0.29 μg/kg,线性范围为0.05~4.05 μg/kg,灵敏度为0.05 μg/kg,添加回收率均大于85%。该法测定玉米赤霉烯酮灵敏度高,重复性好,特异性高,适合用于检测各种玉米油中的玉米赤霉烯酮残留。
[Key word]
[Abstract]
To know the performance of the kit, indirect competitive enzyme-linked immunosorbent assay(ELISA) was used to detect the residues of zearalenone in the 3 kinds of corn oil. The results showed that the linear equation was: y=-2.2x+0.66, r=0.9974, IC50=0.29 μg/kg. Quantitation of zearalenone was linear from 0.05 μg/kg to 4.05 μg/kg. The sensitivity was 0.05 μg/kg. The recovery rates in the corn oil were more than 85%. The sensitivity, repeatability and specificity of the method were satisfactory and could meet the need of detecting the residues of zenralenone in corn oil.
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