[关键词]
[摘要]
巴戟天废弃药渣含有丰富的多糖,故此研究巴戟天药渣中多糖热水提取的最佳工艺条件,为巴戟天废弃药渣的综合利用提供依据。以多糖得率为指标,通过单因素实验研究4个因素:提取温度、提取时间、料液比、提取次数对巴戟天药渣中多糖提取效率的影响。在单因素实验的基础上,通过正交试验对巴戟天热水提取工艺条件进行优化。结果表明,提取温度及提取次数是影响巴戟天多糖提取效率的关键因素,料液比及提取时间是次要因素。最佳工艺条件为:巴戟天药渣在100 ℃水浴中用7倍量的蒸馏水提取2次,每次1 h,在此条件下,巴戟天多糖的得率为15.67%。该工艺条件可重复,多糖得率较高。
[Key word]
[Abstract]
In order to utilize the discarded residue, the extraction conditions for polysaccharides in the discarded residue of Morinda officinalis How were investigated. With the yield of polysaccharides as index, the effects of several factors such as temperature, time, ratio of solid to liquid and times on the extraction of polysaccharides from M. officinalis residue were invstigated using single factor analysis. On the base of the single factor analysis, the optimal the extraction conditions were obtained by orthogonal analysis. The results showed that temperature and times were the major factors which affecting the extraction of polysaccharides, time and ratio of solid to liquid were the secondary ones. The best conditions for extraction were: distilled water extraction time 1 h, temperature 100 ℃, ratio of solid to liquid 1:7 (m/V). and extracting times 2. Under these conditions, the yield of M. officinalis polysaccharides was 15.67%. The process was reproducible with high yield of polysaccharides.
[中图分类号]
[基金项目]
湛江市财政资金科技专项竞争性分配项目(湛科[2010]174号);湛江师范学院博士专项项目(ZL1008);广东省大学生创新创业训练计划项目(1057912006)