[关键词]
[摘要]
1,3-二油酸-2-棕榈酸甘油三酯(OPO)是母乳脂肪中甘油三酯的重要组成成分,可有效改善和保证棕榈酸在婴幼儿体内的吸收和利用。本文对其脂肪酶催化生产进行了简单探索,以三棕榈酸甘油酯(PPP)和油酸(OA)为底物,通过1,3-特异性脂肪酶RMIM催化的酸解反应成功制备了1,3-二油酸-2-棕榈酸甘油三酯。经单因素优化后最佳反应条件为:PPP与油酸摩尔比1:6,加酶量8%,反应温度50 ℃,反应时间6 h,反应产物中脂肪酸组成为:油酸54.86%,sn-2位棕榈酸含量为87.64%。
[Key word]
[Abstract]
1,3-Dioleoyl-2-palmitoylglycerol (OPO) is an important component of triglycerides in human milk fat. The research focused on the optimization of different reaction factors to produce 1,3-dioleoyl-2-palmitoylglycerol as human milk fat substitute catalyzed by the immobilized lipase RMIM with tripalmitin (PPP) and oleic acid (OA) as substrates. The optimum reaction conditions were as follows: the molar ratio of PPP and oleic acid 1:6, the enzyme dosage 8% of the weight of substrates, the reaction temperature 50 ℃ and the reaction time 6 h. In the products, the concentrations of oleic acid and palmitic acid in sn-2 position were 54.86% and 87.64%, respectively, of the total fatty acids.
[中图分类号]
[基金项目]
广东省高新技术产业化-工业攻关项目,新型脂肪酶RML生产OPO(2012B010300008)