选择国内外常用几种抗性淀粉的测定方法进行比较，找出适合天然抗性淀粉含量的测定方法，并对此方法进行优化。通过单因素和正交试验，对原McCleary法中去除可消化淀粉阶段的体系pH、水浴时间和后期的抗性淀粉测定阶段的糖化酶添加量、作用时间、水浴温度等进行优化，实验确定的去除可消化淀粉阶段复合酶溶液的pH为6.0，水浴时间为12 h，后期抗性淀粉测定阶段的糖化酶添加量为300 μL，水浴温度为55 ℃，水浴时间为75 min。并从方法的稳定性和精密度等方面对优化后的测定方法进行评价，最终确定了适合天然抗性淀粉的定量检测方法。

Based on one-factor-at-a-time experiments, the main parameters influencing the quantitive detection of natural resistant starch were optimized by orthogonal test, including reaction time, pH value, reaction temperature, and saccharifying enzyme amount. For the remove of digestible starch, pH of mixed enzyme solution and water-incubation time were 6.0 and 12 h, respectively. For determination of resistant starch, the glucoamylase dosage, water-incubation temperature and time were 300 μL, 55 ℃ and 75 min, respectively.

粤港澳关键领域重点突破项目（200849861007）