[关键词]
[摘要]
按照保健食品安全性毒理学评价程序,采用急性毒性试验、遗传毒性试验(Ames试验、小鼠骨髓微核试验、小鼠精子畸形试验)和30 d喂养试验进行粉葛氨糖复方的安全性评价。结果粉葛氨糖复方对雌、雄昆明种小鼠的最大耐受剂量(MTD)均大于20.0 g/(kg?bw);三项遗传毒性试验(Ames试验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸形试验)结果均为阴性;30 d喂养试验未见大鼠的生长发育、血液学、生化及组织病理学有异常变化。粉葛氨糖复方急性毒性分级属无毒级、无遗传毒性,在本实验剂量范围内,属安全性保健食品。
[Key word]
[Abstract]
According to procedures for toxicological assessment of supplement food, acute toxicity test, genetic toxicity test (Ames test,micronucleus test of born marrow in mice,sperm shape abnormality test in mice) and thirty-day feeding test in rats were conducted to assess toxicological safety of mice and rats fed with Kduzu-glucosamine formula. The results showed that mouse oral LD50 was above 20.0 g/(kg?bw). Ames test and micronucleus test of bone marrow cells and sperm shape abnormality test in mice were all negative. Rats after being fed Kduzu -glucosamine Formula for 30 d with three different doses groups grew well. Hematology and hematology biochemistry index were all normal. No abnormal symptoms were observed after gross anatomy. Kduzu-glucosamine formula belongs to the actual innocuous level and no-genetic toxicity, being used according to recommending amounts is safe.
[中图分类号]
[基金项目]
广东省产学研项目(2010B090400455)