采用高效液相色谱法建立五指毛桃入药膳的HPLC 特征指纹图谱，探讨药膳中可作为评价指标的目标药材。运用HPLC分析五指毛桃药膳的指纹图谱。色谱柱：Waters Shield RP C18柱（250 mm×4.6 mm，5 μm）；流动相为甲醇(A)-0.1%磷酸(B)，梯度洗脱；流速1.0 mL/min；检测波长246 nm；柱温30 ℃，测定10批五指毛桃药膳指纹图谱。在特征指纹图谱中，确定14 个共有峰，其中补骨脂素含量最高；十批样品相似度在0.90~0.99，且方法学考察符合规定的标准。五指毛桃入药膳后可以检测出相关特征成分，可以作为药膳质量评价的对象。食材脂肪酸成分对五指毛桃特征成分的检出影响不大。

The fingerprint of Radix Fici in medicated diet by HPLC was established and the medicinal materials that can be used as evaluation index of medicated diet were explored. Chromatographic conditions of HPLC were as follows：Waters Shield RP C18 column (250 mm×4.6 mm, 5 μm), methanol (A) and 0.1% phosphoric acid (B) as gradient mobile phases, flow rate 1.0 mL/min and detecting wavelength at246 nm, and column temperature 30 ℃. Fingerprint was established with 14 common peaks and determination of Psoralen was the highest. Similarity was in the range of 0.90-0.99 and methodology reached the required standard. Characteristic constituents of Radix Fici could be detected in the medicated diet, which was appropriate for the evaluation index of medicated diet. The impact of fatty acid on detection of characteristic constituents of Radix Fici was small.

番禺区科技计划项目（2010-专-12-11）