[关键词]
[摘要]
以脱色率、多糖保留率及脱蛋白率为指标,筛选合适的树脂对巴戟天多糖脱色脱蛋白,并进行静态及动态的工艺研究。确定最佳的工艺条件是:采用D941树脂,室温下(25 ℃),巴戟天多糖液pH值5.96(原液),以流速2 BV/h进行动态吸附。在此工艺条件下,脱色率为89.87%,多糖保留率81.08 %,脱蛋白率50.75%,表明D941树脂具有良好的脱色效果和一定的脱蛋白能力。
[Key word]
[Abstract]
Suitable resin was chosen, and static and dynamic adsorption experiments were carried out to investigate the decolorization and deproteinization effects of polysaccharides from Morinda officinalis How. using the decolorization rate, polysaccharide retention rate and deproteinization rate as main indeces. The results showed that optimum conditions were as follows: D941 resin, room temperature, pH value of polysaccharide solution 5.96, flow rate 2 BV/h, dynamic adsorption. Under the optimal technique, the decolorization rate, polysaccharide retention rate and deproteinization rate were 89.87%, 81.08% and 50.75%, respectively. In conclusion, D941 resin had good decolorization effect and certain deproteinization effect.
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[基金项目]
湛江市财政资金科技专项竞争性分配项目(湛科[2010]174号);湛江师范学院博士专项项目(ZL1008)