采用响应曲面分析法对超声波提取灵芝多糖的工艺条件进行优化，优化后的工艺为：超声功率320 W，提取温度70 ℃，提取时间34 min，其多糖得率为2.78%，氧自由基吸收能力（ORAC值）为1956 μmol Trolox/g，与理论预测值无显著性差异。因此，采用响应面分析法优化灵芝多糖超声提取工艺，具有较好的可行性。在最佳条件下，制备的灵芝多糖分子量组成为18150 Da（20.07%）和6484 Da（79.93%），大大低于传统水提灵芝多糖的分子量。

The response surface methodology was used to optimize the ultrasonic extraction of Ganoderma lucidum polysaccharides. Design expert analysis indicated that the optimal processing conditions were: ultrasounic power 320 W, temperature 70 ℃ and extraction time 34 min, respectively. The extraction yield and the ORAC value of polysaccharide were 2.78% and 1956 μmol Trolox/g, respectively, which had no significant differences with the predicted values, indicating that the response surface methodology was a reasonable method to optimize the extraction conditions of Ganoderma lucidum polysaccharides. The molecular weights distribution of the polysaccharides under the optimal conditions were 18150 Da (20.07%) and 6484 Da (79.93%), much less than those of the polysaccharides prepared by traditional water extraction.

广东省部产学研项目（2010A090200041）；广东省科技计划项目（2011B030500004）；国家自然科学基金（31101222）；企业横向项目“灵芝多糖构效关系及工艺研究”资助