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[摘要]
用清除1,1-二苯基苦基苯肼(DPPH)自由基的方法对糖蜜抗氧化能力进行评价。结果表明DPPH反应体系吸光度值测定波长为517.0 nm,以浓度为47.32 mg/L(1.2×10-4mol/L)的DPPH自由基清除率为50%时糖蜜浓度值(即IC50值)为评价指标,未经电渗析处理的糖蜜IC50值为0.3752 mg/mL,电渗析处理后糖蜜IC50值为0.2884mg/mL,进而了解到糖蜜电渗析过程对其抗氧化能力影响较小。
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[Abstract]
The antioxidant capacity of molasses was evaluated with organic free radical DPPH scavenging reaction system. The wavelength for the determination of DPPH reaction system by spectrophotometric method was chosen as 517.0 nm and the value of molasses concentration for scavenging half of DPPH (1.2×10-4 mol/L)radica1 (IC50 value) was used as the index to evaluate scavenging capacity. Results showed that IC50 values for molasses with and without electrodialysis treatment were 0.2887 mg/mL and 0.3669 mg/mL, respectively. It was concluded that that the electrodialysis treatment could reduce the antioxidant capacity of molasses.
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