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[摘要]
以咖啡酸作为对照物,构建蒲公英抑菌提取液氯仿萃取部位的高效液相色谱(HPLC)指纹图谱。本实验方法采用HPLC方法:Diamonsil C18(5 μm,4.6 mm×250 mm)色谱柱,流动相采用乙腈-水-10%乙酸溶液三相系统进行梯度洗脱,流速0.7 mL/min,检测波长323 nm,柱温37 ℃。指纹图谱得到12个共有色谱峰,并可根据这些色谱峰来控制蒲公英抑菌提取液的质量。
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[Abstract]
Caffeic acid was used as internal standard for the HPLC fingerprint establishment of chloroform extract from Taraxacum bacteriostatic. HPLC analysis was performed on a Diamonsil C18 column (5 μm, 4.6 mm×250 mm) with the three-phase system of acetonitrile-water-10% acetic acid solution as the mobile phase for gradient elution. The flow rate, detection wavelength and column temperature were 0.7 mL/min, 323 nm and 37 ℃, respectively. 12 Common peaks were showed in the fingerprint, which can be used to control the quantity of the Taraxacum bacteriostatic extraction.
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