[关键词]
[摘要]
基于谷氨酸棒杆菌(Corynebacterium glutamicum)代谢网络和L-组氨酸合成代谢流分析,对L-组氨酸产生菌谷氨酸棒杆菌TQ2223(Phe-,Tyr-,8-AGr,SGr,CINr)进行多次硫酸二乙酯(DES)定向诱变,依次赋予其6-MPr、5-MTr、2-TAr和L-Hisase-(L-组氨酸酶缺陷)遗传标记后得到突变株TL1106(5-MTr,SGr,5-FTr,8-AGr,6-MPr,2-TAr,Hisase-)。经验证突变株TL1106可在10%葡萄糖的发酵培养基上积累L-组氨酸6.0 g/L,比出发菌株提高了2.5倍。结果表明:通过赋予目的遗传标记而改变代谢流分布,能够促进L-组氨酸的合成与积累。
[Key word]
[Abstract]
According to the theory of metabolic flux analysis, TL1106(5-MTr,SGr,5-FTr,8-AGr,6-MPr,2-TAr, Hisase-) was obtained by diethyl sulfate (DES) mutagenesis derived from the original strain Corynebacterium glutamicum TQ2223 (Phe-,Tyr-,8-AGr,SGr,CINr). TL1106 can produce 6.0 g/L of L-Histidine in 10% glucose medium which was 2.5 times higher than that byTQ2223. Accordingly, the metabolic flux of C.glutamicum TQ2223 and its mutants TL1106 was determined, compared and analyzed in L-Histidine synthesis. Therefore, the great change of metabolic flux distribution might be caused by the introduction of genetic markers, which was useful in L-Histidine synthesis.
[中图分类号]
[基金项目]
“十一五”国家科技支撑计划(2008BAI63B01);国家科技重大专项课题(2008ZX09401-05)