[关键词]
[摘要]
本文研究了黑曲霉（ATCC16404）菌丝体真空冷冻干燥的保藏方法，并从培养基和保护剂对冻干菌的影响进行了研究，初步确定了高活菌数的增殖培养基。比较了不同保护剂对菌丝体成活率的影响，确定了最佳保护剂，活菌数达到109 cfu/g。
[Key word]
[Abstract]
In this paper, the preservation of the mycelium of Aspergillus niger by freeze drying was introduced ,and the effects of the cultural medium and freeze-drying protectant were investigated. The enrichment culture medium for Aspergillus niger with high viable cells number and the best freeze-drying protectant were determined, with which the viable cells number of Aspergillus niger treated by freeze drying were 109cfu/g.
[中图分类号]
[基金项目]
国家科技支撑计划（2006BAD05A02-01）、2006年省部产学研结合项目（2006D90204006）资助
