为了对当前爆发流行的高致病性猪繁殖与呼吸系统综合症病毒建立快速准确的检测方法，根据该类病毒在Nsp2基因1594-1680处缺失87个碱基的特点，设计了一对特异性引物和一个Taqman探针，通过对反应条件的优化，建立了荧光定量PCR检测方法。该方法特异性强，灵敏度高，能很好地区分高致病性猪繁殖与呼吸系统综合症病毒和其它病毒，没有发现假阳性和假阴性现象，检测病毒滴度达到1TCID50。用该法对38份疑似样本进行检测，阳性率60.5%，与常规PCR检测法符合率100%。

A method for rapid detection of the highly virulent Porcine Reproductive and Respiratory syndrome virus (H-PRRSV) was developed using fluorescence quantitative PCR assay (FQ-PCR). The Taqman probe and a pair of specific primers and were designed based on Nsp2 gene with 87-base-pairs sequence deletion. Then the FQ-PCR method was developed by optimization of the reaction conditions, which showed high specificity and sensitivity, and well differentiated H-PRRSV form other viruses. 1TCID50/ml of the H-PRRSV could be detected by this method. Besides, similar positive percentages of 38 tissue specimens (60.5%) were found using FQ-PCR assay or conventional PCR assay.