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[摘要]
本文从荔枝果皮中提取纯化得到了高活性多酚氧化酶和高纯度花色苷,在此基础上,研究了花色苷-PPO-表儿茶素之间的相互作用以及花色苷降解速率受各反应物浓度的影响。结果表明,PPO先与表儿茶素发生氧化还原反应,随着大量表儿茶素氧化产物生成,其氧化产物再与花色苷作用,使花色苷降解。花色苷降解速率受各反应物浓度的影响较大。
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[Abstract]
Polyphenol oxidase and anthocyanins with high purity were obtained from Litchi pericarp by modern purification methods. The degradation mechanism of anthocyanins in the presence of (-)-epicatechin and litchi pericarp polyphenol oxidase (PPO) and the effects of the reactant concentration on the anthocyanins degradation rate were studied. The results showed that redox reaction between PPO and (-)-epicatechin occurred firstly and generated lots of (-)-epicatechin oxidation products, which reacted with anthocyanins and resulted in degradation of anthocyanins. Anthocyanins degradation rate was affected obviously by the substrate concentration.
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