通过化学方法制备了环丙沙星的免疫抗原和包被抗原；分别采用碳二亚胺（EDC）法和氯甲酸乙丁酯法把环丙沙星与BSA进行了偶联制备了人工免疫抗原CIP-BSA，EDC法制备了包被抗原CIP-OVA，经PAGE、紫外分光光度法测定了人工合成抗原的偶联比，EDC法和氯甲酸乙丁酯法制得的CIP-BSA两分子结合比率分别为4:1和11:1，CIP-OVA中两分子结合比率为12:1。这为环丙沙星单克隆抗体的制备奠定了基础。

The Ciprofloxacin (CIP) was conjugated directly to bovine serum albumin(BSA) by carbodiimide (EDC) method and isobutyl chloroformate method, giving the immunizing antigen. And the coating antigen, CIP-OVA, was synthesized by EDC method. The conjugated CIP-BSA and CIP-OVA were analyzed by non-denaturing gel electrophoresis and UV spectrophotometry. Results showed that the molecule conjugate ratio of CIP to BSA by EDC method and isobutyl chloroformate method were 4:1 and 11:1, respectively. The molecule conjugate ratio of CIP to OVA was 12:1. The successful synthesis of Ciprofloxacin artificial antigens provided references for establishment of immunoassay of Ciprofloxacin.