通过与一种不同来源的脂肪酶作比较，研究了粗状假丝酵母诱变株Z6产脂肪酶水解不同底物的相对酶活，包括对低级酯、脂肪酸甘油酯和天然油脂的水解。并对粗状假丝酵母Z6产脂肪酶催化水解玉米油和大豆油反应中的pH值、温度、时间、油/水比、乳化剂用量和Ca2+浓度等因素进行了研究，得出pH值为7.5，温度为45 ℃，水解反应时间为21 h，油水比为1/10，乳化剂相对于油的浓度为4%，Ca2+浓度为75 mmol/L时，玉米油和大豆油都有较好的水解率。通过对该酶反应动力学进行研究得出其水解反应的米氏常数Km为12.25×10-3 mol/L，最大反应速率Vmax 为11.14 µmol/min。

The relative activity of a lipase from Candida valida Z6 was studied in hydrolysis of simple esters, fatty acid glycerides and lipidse. For the hydrolysis of corn oil and soybean oil by the lipase, the optimum hydrolysis conditions were: pH value 7.5, temperature 45 ℃, hydrolysis time 21 h, oil/water ratio 1/10, emulsifying agent content of 4%(oil) and Ca2+ concentration of 75 mmol/L. The enzyme kinetics analysis revealed that the Km and Vmax were 12.25×10-3 mol/L and 11.14 µmol/min, respectively.

广东省农业攻关计划（2006B13001001）资助